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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Nostoc sp. C-phycocyanin subunit beta (cpcB) CSB-YP362050NHR
CSB-EP362050NHR
CSB-BP362050NHR
CSB-MP362050NHR
CSB-EP362050NHR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fremyella diplosiphon C-phycocyanin-1 alpha chain (cpcA1) CSB-YP362051FCY
CSB-EP362051FCY
CSB-BP362051FCY
CSB-MP362051FCY
CSB-EP362051FCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. Phycobilisome 8.9 kDa linker polypeptide, phycocyanin-associated, rod (cpcD) CSB-YP362052NHR
CSB-EP362052NHR
CSB-BP362052NHR
CSB-MP362052NHR
CSB-EP362052NHR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fremyella diplosiphon Phycocyanobilin lyase subunit alpha (cpcE) CSB-YP362053FCY
CSB-EP362053FCY
CSB-BP362053FCY
CSB-MP362053FCY
CSB-EP362053FCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Beta-xylosidase (xynB), partial CSB-YP362054BRC
CSB-EP362054BRC
CSB-BP362054BRC
CSB-MP362054BRC
CSB-EP362054BRC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bluetongue virus 10 Core protein VP4 (Segment-4), partial CSB-YP362055BHQ
CSB-EP362055BHQ
CSB-BP362055BHQ
CSB-MP362055BHQ
CSB-EP362055BHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max 30S ribosomal protein S12, chloroplastic (rps12-A) CSB-YP362056GGV
CSB-EP362056GGV
CSB-BP362056GGV
CSB-MP362056GGV
CSB-EP362056GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida maltosa 3-isopropylmalate dehydrogenase (LEU2) CSB-YP362057CZJ
CSB-EP362057CZJ
CSB-BP362057CZJ
CSB-MP362057CZJ
CSB-EP362057CZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus High mobility group protein B1 (HMGB1) CSB-YP362058DXU
CSB-EP362058DXU
CSB-BP362058DXU
CSB-MP362058DXU
CSB-EP362058DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Protein virC1 (virC1) CSB-YP362059AYS
CSB-EP362059AYS
CSB-BP362059AYS
CSB-MP362059AYS
CSB-EP362059AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium radiobacter Limited host range virA protein (virA), partial CSB-YP362060RKV
CSB-EP362060RKV
CSB-BP362060RKV
CSB-MP362060RKV
CSB-EP362060RKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Protein VirB11 (virB11) CSB-YP362061AYS
CSB-EP362061AYS
CSB-BP362061AYS
CSB-MP362061AYS
CSB-EP362061AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium radiobacter Protein parA (parA) CSB-YP362062RKV
CSB-EP362062RKV
CSB-BP362062RKV
CSB-MP362062RKV
CSB-EP362062RKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Ribulose bisphosphate carboxylase small chain 2A, chloroplastic (RBCS-2A) CSB-YP362063SZY
CSB-EP362063SZY
CSB-BP362063SZY
CSB-MP362063SZY
CSB-EP362063SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Chorion protein S36 (Cp36) CSB-YP362064DLU
CSB-EP362064DLU
CSB-BP362064DLU
CSB-MP362064DLU
CSB-EP362064DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Chorion protein S18 (Cp18) CSB-YP362065DLU
CSB-EP362065DLU
CSB-BP362065DLU
CSB-MP362065DLU
CSB-EP362065DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Chorion protein S19 (Cp19) CSB-YP362066DLU
CSB-EP362066DLU
CSB-BP362066DLU
CSB-MP362066DLU
CSB-EP362066DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Larval cuticle protein 3 (Lcp3) CSB-YP362067DLU
CSB-EP362067DLU
CSB-BP362067DLU
CSB-MP362067DLU
CSB-EP362067DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Probable maltase H (LvpH), partial CSB-YP362068DLU
CSB-EP362068DLU
CSB-BP362068DLU
CSB-MP362068DLU
CSB-EP362068DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Maltase A3 (Mal-A3), partial CSB-YP362069DLU
CSB-EP362069DLU
CSB-BP362069DLU
CSB-MP362069DLU
CSB-EP362069DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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