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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella typhimurium 10 kDa chaperonin (groS) CSB-YP363076SXB
CSB-EP363076SXB
CSB-BP363076SXB
CSB-MP363076SXB
CSB-EP363076SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 10 kDa chaperonin (groS) CSB-YP363077SWW
CSB-EP363077SWW
CSB-BP363077SWW
CSB-MP363077SWW
CSB-EP363077SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP363078SWW
CSB-EP363078SWW
CSB-BP363078SWW
CSB-MP363078SWW
CSB-EP363078SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cold shock-like protein CspH (cspH) CSB-YP363079SWW
CSB-EP363079SWW
CSB-BP363079SWW
CSB-MP363079SWW
CSB-EP363079SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cysteine synthase A (cysK) CSB-YP363080SWW
CSB-EP363080SWW
CSB-BP363080SWW
CSB-MP363080SWW
CSB-EP363080SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major curlin subunit (csgA) CSB-YP363081SWW
CSB-EP363081SWW
CSB-BP363081SWW
CSB-MP363081SWW
CSB-EP363081SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis Major curlin subunit (csgA) CSB-YP363082SBG
CSB-EP363082SBG
CSB-BP363082SBG
CSB-MP363082SBG
CSB-EP363082SBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Minor curlin subunit (csgB) CSB-YP363083SBG
CSB-EP363083SBG
CSB-BP363083SBG
CSB-MP363083SBG
CSB-EP363083SBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-alanine--D-alanine ligase A (ddlA) CSB-YP363084SWW
CSB-EP363084SWW
CSB-BP363084SWW
CSB-MP363084SWW
CSB-EP363084SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Diaminopimelate epimerase (dapF) CSB-YP363085SWW
CSB-EP363085SWW
CSB-BP363085SWW
CSB-MP363085SWW
CSB-EP363085SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Inner membrane protein YghB (yghB), partial CSB-YP363086SWW
CSB-EP363086SWW
CSB-BP363086SWW
CSB-MP363086SWW
CSB-EP363086SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uridine phosphorylase (udp) CSB-YP363087SWW
CSB-EP363087SWW
CSB-BP363087SWW
CSB-MP363087SWW
CSB-EP363087SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein impC (impC) CSB-YP363088ENL
CSB-EP363088ENL
CSB-BP363088ENL
CSB-MP363088ENL
CSB-EP363088ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Virulence protein msgA (msgA) CSB-YP363089SXB
CSB-EP363089SXB
CSB-BP363089SXB
CSB-MP363089SXB
CSB-EP363089SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium RNA polymerase-binding transcription factor DksA (dksA) CSB-YP363090SXB
CSB-EP363090SXB
CSB-BP363090SXB
CSB-MP363090SXB
CSB-EP363090SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Chaperone protein DnaJ (dnaJ) CSB-YP363091SXB
CSB-EP363091SXB
CSB-BP363091SXB
CSB-MP363091SXB
CSB-EP363091SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaJ (dnaJ) CSB-YP363092SWW
CSB-EP363092SWW
CSB-BP363092SWW
CSB-MP363092SWW
CSB-EP363092SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Putative protein-disulfide oxidoreductase (STM3194), partial CSB-YP363093SXB1
CSB-EP363093SXB1
CSB-BP363093SXB1
CSB-MP363093SXB1
CSB-EP363093SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Biopolymer transport protein exbB (exbB), partial CSB-YP363094SXB1
CSB-EP363094SXB1
CSB-BP363094SXB1
CSB-MP363094SXB1
CSB-EP363094SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP363095SXB
CSB-EP363095SXB
CSB-BP363095SXB
CSB-MP363095SXB
CSB-EP363095SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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