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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Scyliorhinus canicula Scyliorhinin-2 CSB-YP362463SYA
CSB-EP362463SYA
CSB-BP362463SYA
CSB-MP362463SYA
CSB-EP362463SYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kassina senegalensis Kassinin CSB-YP362464KAA
CSB-EP362464KAA
CSB-BP362464KAA
CSB-MP362464KAA
CSB-EP362464KAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kassina maculata Hylambates kassinin CSB-YP362465KBA
CSB-EP362465KBA
CSB-BP362465KBA
CSB-MP362465KBA
CSB-EP362465KBA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physalaemus fuscomaculatus Physalaemin CSB-YP362466PJC
CSB-EP362466PJC
CSB-BP362466PJC
CSB-MP362466PJC
CSB-EP362466PJC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human hepatitis A virus genotype IB Genome polyprotein, partial CSB-YP362467HJG
CSB-EP362467HJG
CSB-BP362467HJG
CSB-MP362467HJG
CSB-EP362467HJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Chaperone protein DnaJ (dnaJ) CSB-YP362468ENV
CSB-EP362468ENV
CSB-BP362468ENV
CSB-MP362468ENV
CSB-EP362468ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase iron protein 2 (nifH2) CSB-YP362469MEU
CSB-EP362469MEU
CSB-BP362469MEU
CSB-MP362469MEU
CSB-EP362469MEU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Subtilisin-chymotrypsin inhibitor-2B CSB-YP362470HWQ
CSB-EP362470HWQ
CSB-BP362470HWQ
CSB-MP362470HWQ
CSB-EP362470HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (nodM), partial CSB-YP362471RKR
CSB-EP362471RKR
CSB-BP362471RKR
CSB-MP362471RKR
CSB-EP362471RKR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Acyl-CoA reductase (luxC) CSB-YP362472VFD
CSB-EP362472VFD
CSB-BP362472VFD
CSB-MP362472VFD
CSB-EP362472VFD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Ras-like protein 1 (Ras85D) CSB-YP362473DLU
CSB-EP362473DLU
CSB-BP362473DLU
CSB-MP362473DLU
CSB-EP362473DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkylmercury lyase (merB) CSB-YP362474FKZ
CSB-EP362474FKZ
CSB-BP362474FKZ
CSB-MP362474FKZ
CSB-EP362474FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mercuric transport protein (merT), partial CSB-YP362475FKZ1
CSB-EP362475FKZ1
CSB-BP362475FKZ1
CSB-MP362475FKZ1
CSB-EP362475FKZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipase CSB-YP362476PUK
CSB-EP362476PUK
CSB-BP362476PUK
CSB-MP362476PUK
CSB-EP362476PUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photinus pyralis Luciferin 4-monooxygenase, partial CSB-YP362477PID
CSB-EP362477PID
CSB-BP362477PID
CSB-MP362477PID
CSB-EP362477PID-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkylmercury lyase (merB) CSB-YP362478SYN
CSB-EP362478SYN
CSB-BP362478SYN
CSB-MP362478SYN
CSB-EP362478SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Envelope glycoprotein B (gB), partial CSB-YP362479HJX
CSB-BP362479HJX
CSB-MP362479HJX
CSB-EP362479HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lassa virus Pre-glycoprotein polyprotein GP complex (GPC), partial CSB-YP362480LNP2
CSB-BP362480LNP2
CSB-MP362480LNP2
CSB-EP362480LNP2-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabies virus Matrix protein (M) CSB-YP362481RAG
CSB-EP362481RAG
CSB-BP362481RAG
CSB-MP362481RAG
CSB-EP362481RAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium cynomolgi Circumsporozoite protein CSB-YP362482PLJ
CSB-EP362482PLJ
CSB-BP362482PLJ
CSB-MP362482PLJ
CSB-EP362482PLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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