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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Alpha-glucosidase MAL62 (MAL62), partial CSB-YP362091SAC
CSB-EP362091SAC
CSB-BP362091SAC
CSB-MP362091SAC
CSB-EP362091SAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Saccharopepsin (PEP4) CSB-YP362092SVG 500μg/100μg/20μg
Recombinant Saccharomyces cerevisiae Regulatory protein PHO2 (PHO2), partial CSB-YP362093SVG
CSB-EP362093SVG
CSB-BP362093SVG
CSB-MP362093SVG
CSB-EP362093SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA repair and recombination protein PIF1 (PIF1), partial CSB-YP362094SVG
CSB-EP362094SVG
CSB-BP362094SVG
CSB-MP362094SVG
CSB-EP362094SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 40S ribosomal protein S19-A (RPS19A) CSB-YP362095SVG
CSB-EP362095SVG
CSB-BP362095SVG
CSB-MP362095SVG
CSB-EP362095SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Phosphomannomutase (SEC53) CSB-YP362096SVG
CSB-EP362096SVG
CSB-BP362096SVG
CSB-MP362096SVG
CSB-EP362096SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Anthranilate phosphoribosyltransferase (TRP4) CSB-YP362097SVG
CSB-EP362097SVG
CSB-BP362097SVG
CSB-MP362097SVG
CSB-EP362097SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea rRNA adenine N-6-methyltransferase (ermE) CSB-YP362098STB
CSB-EP362098STB
CSB-BP362098STB
CSB-MP362098STB
CSB-EP362098STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mizuhopecten yessoensis Myosin, essential light chain, adductor muscle CSB-YP362099MUD
CSB-EP362099MUD
CSB-BP362099MUD
CSB-MP362099MUD
CSB-EP362099MUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine parvovirus Probable non-capsid protein NP1 CSB-YP362100BKQ
CSB-EP362100BKQ
CSB-BP362100BKQ
CSB-MP362100BKQ
CSB-EP362100BKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine parvovirus Capsid protein VP1, partial CSB-YP362101BKQ
CSB-EP362101BKQ
CSB-BP362101BKQ
CSB-MP362101BKQ
CSB-EP362101BKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human parvovirus B19 Minor capsid protein VP1, partial CSB-YP362102HPM
CSB-MP362102HPM
CSB-EP362102HPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine minute virus Capsid protein VP1, partial CSB-YP362103MKF
CSB-EP362103MKF
CSB-BP362103MKF
CSB-MP362103MKF
CSB-EP362103MKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin, 2Fe-2S CSB-YP362104CMA
CSB-EP362104CMA
CSB-BP362104CMA
CSB-MP362104CMA
CSB-EP362104CMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Allophycocyanin beta chain (apcB) CSB-YP362105AJK
CSB-EP362105AJK
CSB-BP362105AJK
CSB-MP362105AJK
CSB-EP362105AJK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase molybdenum-iron protein alpha chain (nifD) CSB-YP362106DPX
CSB-EP362106DPX
CSB-BP362106DPX
CSB-MP362106DPX
CSB-EP362106DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Chemotaxis response regulator protein-glutamate methylesterase (cheB) CSB-YP362107ENV
CSB-EP362107ENV
CSB-BP362107ENV
CSB-MP362107ENV
CSB-EP362107ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Mitosis inducer protein kinase cdr1 (cdr1), partial CSB-YP362108SXV
CSB-EP362108SXV
CSB-BP362108SXV
CSB-MP362108SXV
CSB-EP362108SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces marxianus Beta-glucosidase, partial CSB-YP362109KAN
CSB-EP362109KAN
CSB-BP362109KAN
CSB-MP362109KAN
CSB-EP362109KAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Acetolactate synthase catalytic subunit, mitochondrial (ILV2), partial CSB-YP362110SVG
CSB-EP362110SVG
CSB-BP362110SVG
CSB-MP362110SVG
CSB-EP362110SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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