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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Candida tropicalis Peroxisomal catalase (POX9) CSB-YP362236CZN
CSB-EP362236CZN
CSB-BP362236CZN
CSB-MP362236CZN
CSB-EP362236CZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Iron (3+)-hydroxamate-binding protein fhuD CSB-YP362237ENV
CSB-EP362237ENV
CSB-BP362237ENV
CSB-MP362237ENV
CSB-EP362237ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Photosystem Q (B) protein 1, partial CSB-YP362238SSQ1
CSB-EP362238SSQ1
CSB-BP362238SSQ1
CSB-MP362238SSQ1
CSB-EP362238SSQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Actin-3 (act3) CSB-YP362239DKK
CSB-EP362239DKK
CSB-BP362239DKK
CSB-MP362239DKK
CSB-EP362239DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus RNA-directed RNA polymerase catalytic subunit (PB1), partial CSB-YP362240IJK
CSB-EP362240IJK
CSB-BP362240IJK
CSB-MP362240IJK
CSB-EP362240IJK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum Hypoxanthine-guanine-xanthine phosphoribosyltransferase (LACZ) CSB-YP362241EWT
CSB-EP362241EWT
CSB-BP362241EWT
CSB-MP362241EWT
CSB-EP362241EWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudopleuronectes americanus Ice-structuring protein 2A7 CSB-YP362242FGV
CSB-EP362242FGV
CSB-BP362242FGV
CSB-MP362242FGV
CSB-EP362242FGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori Actin, muscle-type A2 CSB-YP362243BTT
CSB-EP362243BTT
CSB-BP362243BTT
CSB-MP362243BTT
CSB-EP362243BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Ferredoxin, chloroplastic (PETF) CSB-YP362244DSJ
CSB-EP362244DSJ
CSB-BP362244DSJ
CSB-MP362244DSJ
CSB-EP362244DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus 50S ribosomal protein L36 (rpmJ) CSB-YP362245GFM
CSB-EP362245GFM
CSB-BP362245GFM
CSB-MP362245GFM
CSB-EP362245GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus 50S ribosomal protein L17 (rplQ) CSB-YP362246GFM
CSB-EP362246GFM
CSB-BP362246GFM
CSB-MP362246GFM
CSB-EP362246GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stoichactis helianthus Sticholysin-2 CSB-YP362247SMA
CSB-EP362247SMA
CSB-BP362247SMA
CSB-MP362247SMA
CSB-EP362247SMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ascaris suum Chymotrypsin/elastase isoinhibitor 1 CSB-YP362248DOS
CSB-EP362248DOS
CSB-BP362248DOS
CSB-MP362248DOS
CSB-EP362248DOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucurbita maxima Trypsin inhibitor 4 CSB-YP362249DZK
CSB-EP362249DZK
CSB-BP362249DZK
CSB-MP362249DZK
CSB-EP362249DZK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida XylDLEGF operon transcriptional activator (xylS) CSB-YP362250FFZ
CSB-EP362250FFZ
CSB-BP362250FFZ
CSB-MP362250FFZ
CSB-EP362250FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Neprilysin (Mme), partial CSB-YP362251RA
CSB-EP362251RA
CSB-BP362251RA
CSB-MP362251RA
CSB-EP362251RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Guanine nucleotide exchange factor LTE1 (LTE1), partial CSB-YP362252SVG
CSB-EP362252SVG
CSB-BP362252SVG
CSB-MP362252SVG
CSB-EP362252SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Spore germination protein A1 (gerAA), partial CSB-YP362253BRJ1
CSB-EP362253BRJ1
CSB-BP362253BRJ1
CSB-MP362253BRJ1
CSB-EP362253BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Spore germination protein A3 (gerAC) CSB-YP362254BRJ
CSB-EP362254BRJ
CSB-BP362254BRJ
CSB-MP362254BRJ
CSB-EP362254BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Alginate biosynthesis protein AlgA (algA) CSB-YP362255EZX
CSB-EP362255EZX
CSB-BP362255EZX
CSB-MP362255EZX
CSB-EP362255EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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