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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cat Double-headed protease inhibitor, submandibular gland CSB-YP362441CA
CSB-EP362441CA
CSB-BP362441CA
CSB-MP362441CA
CSB-EP362441CA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Echo 9 virus Genome polyprotein CSB-YP362442EAM
CSB-EP362442EAM
CSB-BP362442EAM
CSB-MP362442EAM
CSB-EP362442EAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human parainfluenza 3 virus Hemagglutinin-neuraminidase (HN), partial CSB-YP362443HPI1
CSB-EP362443HPI1
CSB-BP362443HPI1
CSB-MP362443HPI1
CSB-EP362443HPI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Aspartokinase II operon leader peptide (ask) CSB-YP362444BRJ
CSB-EP362444BRJ
CSB-BP362444BRJ
CSB-MP362444BRJ
CSB-EP362444BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Nitrate reductase [NADH] 2 (NIA2), partial CSB-YP362445NHE
CSB-EP362445NHE
CSB-BP362445NHE
CSB-MP362445NHE
CSB-EP362445NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Arginine attenuator peptide (YOR302W) CSB-YP362447SVG
CSB-EP362447SVG
CSB-BP362447SVG
CSB-MP362447SVG
CSB-EP362447SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays 30S ribosomal protein S14, chloroplastic (rps14) CSB-YP362448ZAX
CSB-EP362448ZAX
CSB-BP362448ZAX
CSB-MP362448ZAX
CSB-EP362448ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays 50S ribosomal protein L14, chloroplastic (rpl14) CSB-YP362449ZAX
CSB-EP362449ZAX
CSB-BP362449ZAX
CSB-MP362449ZAX
CSB-EP362449ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Tubulin alpha chain (tuba) CSB-YP362450XBE
CSB-EP362450XBE
CSB-BP362450XBE
CSB-MP362450XBE
CSB-EP362450XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Guanine nucleotide-binding protein alpha-1 subunit (GPA1) CSB-YP362451SVG
CSB-EP362451SVG
CSB-BP362451SVG
CSB-MP362451SVG
CSB-EP362451SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat UDP-glucuronosyltransferase 2B2 (Ugt2b), partial CSB-YP362452RA1
CSB-EP362452RA1
CSB-BP362452RA1
CSB-MP362452RA1
CSB-EP362452RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nycticebus coucang LINE-1 reverse transcriptase homolog, partial CSB-YP362454NIC
CSB-EP362454NIC
CSB-BP362454NIC
CSB-MP362454NIC
CSB-EP362454NIC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Colicin V production protein (cvpA), partial CSB-YP362455ENV1
CSB-EP362455ENV1
CSB-BP362455ENV1
CSB-MP362455ENV1
CSB-EP362455ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DsdX permease (dsdX), partial CSB-YP362456ENV
CSB-EP362456ENV
CSB-BP362456ENV
CSB-MP362456ENV
CSB-EP362456ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage Mu DNA circularization protein (N) CSB-YP362457EDA
CSB-EP362457EDA
CSB-BP362457EDA
CSB-MP362457EDA
CSB-EP362457EDA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rubella virus Structural polyprotein, partial CSB-YP362458RHT1
CSB-EP362458RHT1
CSB-BP362458RHT1
CSB-MP362458RHT1
CSB-EP362458RHT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum Merozoite surface protein 1 (MSP-1), partial CSB-YP362459PMA
CSB-EP362459PMA
CSB-BP362459PMA
CSB-MP362459PMA
CSB-EP362459PMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human U2 small nuclear ribonucleoprotein B' (SNRPB2) CSB-YP362460HU
CSB-BP362460HU
CSB-MP362460HU
CSB-EP362460HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein H2 (VACWR100), partial CSB-YP362461VAI1
CSB-EP362461VAI1
CSB-BP362461VAI1
CSB-MP362461VAI1
CSB-EP362461VAI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aqualysin-1 (pstI) CSB-YP362462TNN
CSB-EP362462TNN
CSB-BP362462TNN
CSB-MP362462TNN
CSB-EP362462TNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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