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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas sp. Naphthalene 1,2-dioxygenase system ferredoxin subunit (doxA) CSB-YP363055PVJ
CSB-EP363055PVJ
CSB-BP363055PVJ
CSB-MP363055PVJ
CSB-EP363055PVJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Probable transport protein YifK (yifK) CSB-YP363056SXB
CSB-EP363056SXB
CSB-BP363056SXB
CSB-MP363056SXB
CSB-EP363056SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium High-affinity branched-chain amino acid transport ATP-binding protein LivF (livF) CSB-YP363057SXB
CSB-EP363057SXB
CSB-BP363057SXB
CSB-MP363057SXB
CSB-EP363057SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium High-affinity branched-chain amino acid transport ATP-binding protein LivG (livG) CSB-YP363058SXB
CSB-EP363058SXB
CSB-BP363058SXB
CSB-MP363058SXB
CSB-EP363058SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium UvrABC system protein A (uvrA) CSB-YP363059SXB
CSB-EP363059SXB
CSB-BP363059SXB
CSB-MP363059SXB
CSB-EP363059SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Probable ubiquinone biosynthesis protein UbiB (ubiB) CSB-YP363060SXB
CSB-EP363060SXB
CSB-BP363060SXB
CSB-MP363060SXB
CSB-EP363060SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acyl-CoA thioester hydrolase YciA (yciA), partial CSB-YP363061SWW
CSB-EP363061SWW
CSB-BP363061SWW
CSB-MP363061SWW
CSB-EP363061SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alanine racemase, biosynthetic (alr) CSB-YP363062SWW
CSB-EP363062SWW
CSB-BP363062SWW
CSB-MP363062SWW
CSB-EP363062SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adenylate cyclase (cyaA), partial CSB-YP363063SWW
CSB-EP363063SWW
CSB-BP363063SWW
CSB-MP363063SWW
CSB-EP363063SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exodeoxyribonuclease III (xthA) CSB-YP363064SWW
CSB-EP363064SWW
CSB-BP363064SWW
CSB-MP363064SWW
CSB-EP363064SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arginine repressor (argR) CSB-YP363066SWW
CSB-EP363066SWW
CSB-BP363066SWW
CSB-MP363066SWW
CSB-EP363066SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive (aroF) CSB-YP363067SWW
CSB-EP363067SWW
CSB-BP363067SWW
CSB-MP363067SWW
CSB-EP363067SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase epsilon chain (atpC) CSB-YP363068SWW
CSB-EP363068SWW
CSB-BP363068SWW
CSB-MP363068SWW
CSB-EP363068SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable ATP synthase SpaL (spaL) CSB-YP363069SWW
CSB-EP363069SWW
CSB-BP363069SWW
CSB-MP363069SWW
CSB-EP363069SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable ATP synthase SpaL/MxiB (spaL) CSB-YP363070SGH
CSB-EP363070SGH
CSB-BP363070SGH
CSB-MP363070SGH
CSB-EP363070SGH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP363071SWW
CSB-EP363071SWW
CSB-BP363071SWW
CSB-MP363071SWW
CSB-EP363071SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell division protein FtsP (ftsP) CSB-YP363072SWW
CSB-EP363072SWW
CSB-BP363072SWW
CSB-MP363072SWW
CSB-EP363072SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propanediol utilization protein PduA (pduA) CSB-YP363073SWW
CSB-EP363073SWW
CSB-BP363073SWW
CSB-MP363073SWW
CSB-EP363073SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ethanolamine utilization protein EutL (eutL) CSB-YP363074SWW
CSB-EP363074SWW
CSB-BP363074SWW
CSB-MP363074SWW
CSB-EP363074SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium 60 kDa chaperonin (groL), partial CSB-YP363075SXB
CSB-EP363075SXB
CSB-BP363075SXB
CSB-MP363075SXB
CSB-EP363075SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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