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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas putida Amino-acid acetyltransferase (argA) CSB-YP363015FFZ
CSB-EP363015FFZ
CSB-BP363015FFZ
CSB-MP363015FFZ
CSB-EP363015FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 3-oxoadipate CoA-transferase subunit B (pcaJ) CSB-YP363016FFZ
CSB-EP363016FFZ
CSB-BP363016FFZ
CSB-MP363016FFZ
CSB-EP363016FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida ATP synthase protein I (atpI), partial CSB-YP363017FFZ1
CSB-EP363017FFZ1
CSB-BP363017FFZ1
CSB-MP363017FFZ1
CSB-EP363017FFZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cold shock protein CapB (capB) CSB-YP363018PUK
CSB-EP363018PUK
CSB-BP363018PUK
CSB-MP363018PUK
CSB-EP363018PUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. 1,2-dihydroxynaphthalene dioxygenase (doxG) CSB-YP363019PVJ
CSB-EP363019PVJ
CSB-BP363019PVJ
CSB-MP363019PVJ
CSB-EP363019PVJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Naphthalene 1,2-dioxygenase subunit alpha (ndoB) CSB-YP363020FFZ
CSB-EP363020FFZ
CSB-BP363020FFZ
CSB-MP363020FFZ
CSB-EP363020FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Naphthalene 1,2-dioxygenase subunit beta (ndoC) CSB-YP363021FFZ
CSB-EP363021FFZ
CSB-BP363021FFZ
CSB-MP363021FFZ
CSB-EP363021FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Carboxymethylenebutenolidase (clcD) CSB-YP363022FFZ
CSB-EP363022FFZ
CSB-BP363022FFZ
CSB-MP363022FFZ
CSB-EP363022FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP363023FGB
CSB-EP363023FGB
CSB-BP363023FGB
CSB-MP363023FGB
CSB-EP363023FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida DNA primase (dnaG), partial CSB-YP363024FFZ
CSB-EP363024FFZ
CSB-BP363024FFZ
CSB-MP363024FFZ
CSB-EP363024FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida DNA polymerase III subunit beta (dnaN) CSB-YP363025FGB
CSB-EP363025FGB
CSB-BP363025FGB
CSB-MP363025FGB
CSB-EP363025FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP363026FFZ
CSB-EP363026FFZ
CSB-BP363026FFZ
CSB-MP363026FFZ
CSB-EP363026FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Integration host factor subunit alpha (ihfA) CSB-YP363027FGB
CSB-EP363027FGB
CSB-BP363027FGB
CSB-MP363027FGB
CSB-EP363027FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Integration host factor subunit beta (ihfB) CSB-YP363028FFZ
CSB-EP363028FFZ
CSB-BP363028FFZ
CSB-MP363028FFZ
CSB-EP363028FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Uncharacterized protein PP_1224 (PP_1224) CSB-YP363029FGB
CSB-EP363029FGB
CSB-BP363029FGB
CSB-MP363029FGB
CSB-EP363029FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transposase for insertion sequences IS1326/IS1353 (istA), partial CSB-YP363030SZB
CSB-EP363030SZB
CSB-BP363030SZB
CSB-MP363030SZB
CSB-EP363030SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Adenylate kinase (adk) CSB-YP363031FFZ
CSB-EP363031FFZ
CSB-BP363031FFZ
CSB-MP363031FFZ
CSB-EP363031FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Peptidoglycan-associated lipoprotein (pal) CSB-YP363032FGB
CSB-EP363032FGB
CSB-BP363032FGB
CSB-MP363032FGB
CSB-EP363032FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Membrane protein insertase YidC (yidC), partial CSB-YP363033FGB1
CSB-EP363033FGB1
CSB-BP363033FGB1
CSB-MP363033FGB1
CSB-EP363033FGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. Trans-O-hydroxybenzylidenepyruvate hydratase-aldolase (doxI) CSB-YP363034PVJ
CSB-EP363034PVJ
CSB-BP363034PVJ
CSB-MP363034PVJ
CSB-EP363034PVJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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