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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Capsule polysaccharide export outer membrane protein CtrA (ctrA), partial CSB-YP362995NBJ
CSB-EP362995NBJ
CSB-BP362995NBJ
CSB-MP362995NBJ
CSB-EP362995NBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein AvrBs1.1 (avrBs1.1) CSB-YP362996XAK
CSB-EP362996XAK
CSB-BP362996XAK
CSB-MP362996XAK
CSB-EP362996XAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant FK506-binding protein (fbp) CSB-YP362997NGI
CSB-EP362997NGI
CSB-BP362997NGI
CSB-MP362997NGI
CSB-EP362997NGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas axonopodis pv. citri Protein RecA (recA) CSB-YP362998XAD
CSB-EP362998XAD
CSB-BP362998XAD
CSB-MP362998XAD
CSB-EP362998XAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas axonopodis pv. citri Regulatory protein recX (recX) CSB-YP362999XAD
CSB-EP362999XAD
CSB-BP362999XAD
CSB-MP362999XAD
CSB-EP362999XAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L7/L12 (rplL) CSB-YP363000NGC
CSB-EP363000NGC
CSB-BP363000NGC
CSB-MP363000NGC
CSB-EP363000NGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A 50S ribosomal protein L7/L12 (rplL) CSB-YP363001NGF
CSB-EP363001NGF
CSB-BP363001NGF
CSB-MP363001NGF
CSB-EP363001NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 30S ribosomal protein S11 (rpsK) CSB-YP363002XAY
CSB-EP363002XAY
CSB-BP363002XAY
CSB-MP363002XAY
CSB-EP363002XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 30S ribosomal protein S12 (rpsL) CSB-YP363003HUV
CSB-EP363003HUV
CSB-BP363003HUV
CSB-MP363003HUV
CSB-EP363003HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A 30S ribosomal protein S18 (rpsR) CSB-YP363004NGF
CSB-EP363004NGF
CSB-BP363004NGF
CSB-MP363004NGF
CSB-EP363004NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S18 (rpsR) CSB-YP363005NFZ
CSB-EP363005NFZ
CSB-BP363005NFZ
CSB-MP363005NFZ
CSB-EP363005NFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas axonopodis pv. citri 30S ribosomal protein S4 (rpsD) CSB-YP363006XAD
CSB-EP363006XAD
CSB-BP363006XAD
CSB-MP363006XAD
CSB-EP363006XAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas axonopodis pv. citri DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP363007XAD
CSB-EP363007XAD
CSB-BP363007XAD
CSB-MP363007XAD
CSB-EP363007XAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Major ferric iron-binding protein (fbpA) CSB-YP363008NGG
CSB-EP363008NGG
CSB-BP363008NGG
CSB-MP363008NGG
CSB-EP363008NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Phosphoheptose isomerase (gmhA) CSB-YP363009NGG
CSB-EP363009NGG
CSB-BP363009NGG
CSB-MP363009NGG
CSB-EP363009NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B SsrA-binding protein (smpB) CSB-YP363010NGG
CSB-EP363010NGG
CSB-BP363010NGG
CSB-MP363010NGG
CSB-EP363010NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Putative HTH-type transcriptional regulator NMB1378 (NMB1378) CSB-YP363011NGG
CSB-EP363011NGG
CSB-BP363011NGG
CSB-MP363011NGG
CSB-EP363011NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B UPF0271 protein rni3 (rni3) CSB-YP363012NGG
CSB-EP363012NGG
CSB-BP363012NGG
CSB-MP363012NGG
CSB-EP363012NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP363013NGG
CSB-EP363013NGG
CSB-BP363013NGG
CSB-MP363013NGG
CSB-EP363013NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Penicillin-binding protein 1A (mrcA), partial CSB-YP363014NGG
CSB-BP363014NGG
CSB-MP363014NGG
CSB-EP363014NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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