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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rat Synaptotagmin-12 (Syt12), partial CSB-YP023032RA1
CSB-EP023032RA1
CSB-BP023032RA1
CSB-MP023032RA1
CSB-EP023032RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 (Enpp3), partial CSB-YP007681RA
CSB-EP007681RA
CSB-BP007681RA
CSB-MP007681RA
CSB-EP007681RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Ras and Rab interactor 1 (Rin1) CSB-YP019726RA
CSB-EP019726RA
CSB-BP019726RA
CSB-MP019726RA
CSB-EP019726RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Smoothened homolog (Smo), partial CSB-YP021841RA1
CSB-EP021841RA1
CSB-BP021841RA1
CSB-MP021841RA1
CSB-EP021841RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Alpha-1A adrenergic receptor (Adra1a), partial CSB-YP001385MO1
CSB-EP001385MO1
CSB-BP001385MO1
CSB-MP001385MO1
CSB-EP001385MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Calcium/calmodulin-dependent protein kinase kinase 1 (Camkk1) CSB-YP004472RA
CSB-EP004472RA
CSB-BP004472RA
CSB-MP004472RA
CSB-EP004472RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Metabotropic glutamate receptor 1 (Grm1), partial CSB-YP009931MO
CSB-EP009931MO
CSB-BP009931MO
CSB-MP009931MO
CSB-EP009931MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse ALK tyrosine kinase receptor (Alk), partial CSB-YP001606MO
CSB-EP001606MO
CSB-BP001606MO
CSB-MP001606MO
CSB-EP001606MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Proline-serine-threonine phosphatase-interacting protein 1 (Pstpip1) CSB-YP018944MO
CSB-EP018944MO
CSB-BP018944MO
CSB-MP018944MO
CSB-EP018944MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Disks large-associated protein 1 (Dlgap1), partial CSB-YP006940RA
CSB-EP006940RA
CSB-BP006940RA
CSB-MP006940RA
CSB-EP006940RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Long-chain fatty acid transport protein 1 (Slc27a1), partial CSB-YP021533RA1
CSB-EP021533RA1
CSB-BP021533RA1
CSB-MP021533RA1
CSB-EP021533RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse A disintegrin and metalloproteinase with thrombospondin motifs 1 (Adamts1) CSB-YP001298MO
CSB-EP001298MO
CSB-BP001298MO
CSB-MP001298MO
CSB-EP001298MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Dimethylaniline monooxygenase [N-oxide-forming] 5 (Fmo5) CSB-YP008751MO
CSB-EP008751MO
CSB-BP008751MO
CSB-MP008751MO
CSB-EP008751MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Exocyst complex component 5 (Exoc5) CSB-YP007883RA
CSB-EP007883RA
CSB-BP007883RA
CSB-MP007883RA
CSB-EP007883RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Uracil-DNA glycosylase (Ung) CSB-YP025641MO
CSB-EP025641MO
CSB-BP025641MO
CSB-MP025641MO
CSB-EP025641MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Mannan-binding lectin serine protease 1 (Masp1) CSB-YP013508MO
CSB-EP013508MO
CSB-BP013508MO
CSB-MP013508MO
CSB-EP013508MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Enteropeptidase (TMPRSS15), partial CSB-YP018824HU1
CSB-EP018824HU1
CSB-BP018824HU1
CSB-MP018824HU1
CSB-EP018824HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Amyloid beta A4 precursor protein-binding family A member 2 (Apba2) CSB-YP001887MO
CSB-EP001887MO
CSB-BP001887MO
CSB-MP001887MO
CSB-EP001887MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine E-selectin (SELE), partial CSB-YP020975BO1
CSB-EP020975BO1
CSB-BP020975BO1
CSB-MP020975BO1
CSB-EP020975BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Complement component C8 alpha chain (C8A), partial CSB-YP004194RB
CSB-EP004194RB
CSB-BP004194RB
CSB-MP004194RB
CSB-EP004194RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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