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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Desulfovibrio vulgaris subsp. vulgaris Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP380710DHY
CSB-EP380710DHY
CSB-BP380710DHY
CSB-MP380710DHY
CSB-EP380710DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP380711DHY
CSB-EP380711DHY
CSB-BP380711DHY
CSB-MP380711DHY
CSB-EP380711DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP380712DHY
CSB-EP380712DHY
CSB-BP380712DHY
CSB-MP380712DHY
CSB-EP380712DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP380713DHY
CSB-EP380713DHY
CSB-BP380713DHY
CSB-MP380713DHY
CSB-EP380713DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris Undecaprenyl-diphosphatase (uppP), partial CSB-YP380714DHY1
CSB-EP380714DHY1
CSB-BP380714DHY1
CSB-MP380714DHY1
CSB-EP380714DHY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP380715DHY
CSB-EP380715DHY
CSB-BP380715DHY
CSB-MP380715DHY
CSB-EP380715DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris UvrABC system protein B (uvrB), partial CSB-YP380716DHY
CSB-EP380716DHY
CSB-BP380716DHY
CSB-MP380716DHY
CSB-EP380716DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP380717DHY
CSB-EP380717DHY
CSB-BP380717DHY
CSB-MP380717DHY
CSB-EP380717DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP380718DHY
CSB-EP380718DHY
CSB-BP380718DHY
CSB-MP380718DHY
CSB-EP380718DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP380719DHY
CSB-EP380719DHY
CSB-BP380719DHY
CSB-MP380719DHY
CSB-EP380719DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris Trigger factor (tig) CSB-YP380720DHY
CSB-EP380720DHY
CSB-BP380720DHY
CSB-MP380720DHY
CSB-EP380720DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris 30S ribosomal protein S13 (rpsM) CSB-YP380721DHY
CSB-EP380721DHY
CSB-BP380721DHY
CSB-MP380721DHY
CSB-EP380721DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris 50S ribosomal protein L18 (rplR) CSB-YP380722DHY
CSB-EP380722DHY
CSB-BP380722DHY
CSB-MP380722DHY
CSB-EP380722DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris 30S ribosomal protein S17 (rpsQ) CSB-YP380723DHY
CSB-EP380723DHY
CSB-BP380723DHY
CSB-MP380723DHY
CSB-EP380723DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris 50S ribosomal protein L16 (rplP) CSB-YP380724DHY
CSB-EP380724DHY
CSB-BP380724DHY
CSB-MP380724DHY
CSB-EP380724DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris 50S ribosomal protein L4 (rplD) CSB-YP380725DHY
CSB-EP380725DHY
CSB-BP380725DHY
CSB-MP380725DHY
CSB-EP380725DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris Phosphoglucosamine mutase (glmM) CSB-YP380726DHY
CSB-EP380726DHY
CSB-BP380726DHY
CSB-MP380726DHY
CSB-EP380726DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris Arginine--tRNA ligase (argS), partial CSB-YP380727DHY
CSB-EP380727DHY
CSB-BP380727DHY
CSB-MP380727DHY
CSB-EP380727DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP380728DHY
CSB-EP380728DHY
CSB-BP380728DHY
CSB-MP380728DHY
CSB-EP380728DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris Leucine--tRNA ligase (leuS), partial CSB-YP380729DHY
CSB-EP380729DHY
CSB-BP380729DHY
CSB-MP380729DHY
CSB-EP380729DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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