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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Halorhodospira halophila Octanoyltransferase (lipB) CSB-YP380510HSG
CSB-EP380510HSG
CSB-BP380510HSG
CSB-MP380510HSG
CSB-EP380510HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Ribulose bisphosphate carboxylase large chain (cbbL) CSB-YP380511HSG
CSB-EP380511HSG
CSB-BP380511HSG
CSB-MP380511HSG
CSB-EP380511HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Sec-independent protein translocase protein TatA (tatA), partial CSB-YP380512HSG
CSB-EP380512HSG
CSB-BP380512HSG
CSB-MP380512HSG
CSB-EP380512HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP380513HSG
CSB-EP380513HSG
CSB-BP380513HSG
CSB-MP380513HSG
CSB-EP380513HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila D-tyrosyl-tRNA (Tyr) deacylase CSB-YP380514HSG
CSB-EP380514HSG
CSB-BP380514HSG
CSB-MP380514HSG
CSB-EP380514HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Glycogen synthase (glgA) CSB-YP380515HSG
CSB-EP380515HSG
CSB-BP380515HSG
CSB-MP380515HSG
CSB-EP380515HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Diaminopimelate epimerase (dapF) CSB-YP380516HSG
CSB-EP380516HSG
CSB-BP380516HSG
CSB-MP380516HSG
CSB-EP380516HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Protein-export protein secB (secB) CSB-YP380517HSG
CSB-EP380517HSG
CSB-BP380517HSG
CSB-MP380517HSG
CSB-EP380517HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP380518HSG
CSB-EP380518HSG
CSB-BP380518HSG
CSB-MP380518HSG
CSB-EP380518HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Membrane protein insertase YidC (yidC), partial CSB-YP380519HSG1
CSB-EP380519HSG1
CSB-BP380519HSG1
CSB-MP380519HSG1
CSB-EP380519HSG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila tRNA modification GTPase MnmE (mnmE) CSB-YP380520HSG
CSB-EP380520HSG
CSB-BP380520HSG
CSB-MP380520HSG
CSB-EP380520HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Phosphate acyltransferase (plsX) CSB-YP380521HSG
CSB-EP380521HSG
CSB-BP380521HSG
CSB-MP380521HSG
CSB-EP380521HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP380522HSG
CSB-EP380522HSG
CSB-BP380522HSG
CSB-MP380522HSG
CSB-EP380522HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP380523HSG
CSB-EP380523HSG
CSB-BP380523HSG
CSB-MP380523HSG
CSB-EP380523HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP380524HSG
CSB-EP380524HSG
CSB-BP380524HSG
CSB-MP380524HSG
CSB-EP380524HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Glutamate--tRNA ligase 2 (gltX2) CSB-YP380525HSG
CSB-EP380525HSG
CSB-BP380525HSG
CSB-MP380525HSG
CSB-EP380525HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Siroheme synthase 1 (cysG1) CSB-YP380526HSG
CSB-EP380526HSG
CSB-BP380526HSG
CSB-MP380526HSG
CSB-EP380526HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP380527HSG
CSB-EP380527HSG
CSB-BP380527HSG
CSB-MP380527HSG
CSB-EP380527HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP380528HSG
CSB-EP380528HSG
CSB-BP380528HSG
CSB-MP380528HSG
CSB-EP380528HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Putative arginyl-tRNA--protein transferase (ate) CSB-YP380529HSG
CSB-EP380529HSG
CSB-BP380529HSG
CSB-MP380529HSG
CSB-EP380529HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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