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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 30S ribosomal protein S7 (rpsG) CSB-YP380310CWF
CSB-EP380310CWF
CSB-BP380310CWF
CSB-MP380310CWF
CSB-EP380310CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 Chaperone protein htpG (htpG), partial CSB-YP380311CWF
CSB-EP380311CWF
CSB-BP380311CWF
CSB-MP380311CWF
CSB-EP380311CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 Elongation factor P (efp) CSB-YP380312CWF
CSB-EP380312CWF
CSB-BP380312CWF
CSB-MP380312CWF
CSB-EP380312CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 RNA pyrophosphohydrolase (rppH) CSB-YP380313CWF
CSB-EP380313CWF
CSB-BP380313CWF
CSB-MP380313CWF
CSB-EP380313CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 DNA ligase (ligA), partial CSB-YP380314CWF
CSB-EP380314CWF
CSB-BP380314CWF
CSB-MP380314CWF
CSB-EP380314CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP380315CWF
CSB-EP380315CWF
CSB-BP380315CWF
CSB-MP380315CWF
CSB-EP380315CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 Flagellar L-ring protein (flgH) CSB-YP380316CWF
CSB-EP380316CWF
CSB-BP380316CWF
CSB-MP380316CWF
CSB-EP380316CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 Ribosome maturation factor RimM (rimM) CSB-YP380317CWF
CSB-EP380317CWF
CSB-BP380317CWF
CSB-MP380317CWF
CSB-EP380317CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 NH (3)-dependent NAD (+) synthetase CSB-YP380318CWF
CSB-EP380318CWF
CSB-BP380318CWF
CSB-MP380318CWF
CSB-EP380318CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 Glutamate--tRNA ligase 1 (gltX1) CSB-YP380319CWF
CSB-EP380319CWF
CSB-BP380319CWF
CSB-MP380319CWF
CSB-EP380319CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 DNA translocase FtsK (ftsK), partial CSB-YP380320CWF
CSB-EP380320CWF
CSB-BP380320CWF
CSB-MP380320CWF
CSB-EP380320CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 Alanine racemase (alr) CSB-YP380321CWF
CSB-EP380321CWF
CSB-BP380321CWF
CSB-MP380321CWF
CSB-EP380321CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 Chemotaxis protein methyltransferase (cheR) CSB-YP380322CWF
CSB-EP380322CWF
CSB-BP380322CWF
CSB-MP380322CWF
CSB-EP380322CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 tRNA modification GTPase MnmE (mnmE) CSB-YP380323CWF
CSB-EP380323CWF
CSB-BP380323CWF
CSB-MP380323CWF
CSB-EP380323CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 tRNA (mo5U34)-methyltransferase CSB-YP380324CWF
CSB-EP380324CWF
CSB-BP380324CWF
CSB-MP380324CWF
CSB-EP380324CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP380325CWF
CSB-EP380325CWF
CSB-BP380325CWF
CSB-MP380325CWF
CSB-EP380325CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 30S ribosomal protein S18 (rpsR) CSB-YP380326CWF
CSB-EP380326CWF
CSB-BP380326CWF
CSB-MP380326CWF
CSB-EP380326CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 Thiamine-phosphate synthase (thiE) CSB-YP380327CWF
CSB-EP380327CWF
CSB-BP380327CWF
CSB-MP380327CWF
CSB-EP380327CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 SsrA-binding protein (smpB) CSB-YP380328CWF
CSB-EP380328CWF
CSB-BP380328CWF
CSB-MP380328CWF
CSB-EP380328CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 Arginine--tRNA ligase (argS), partial CSB-YP380329CWF
CSB-EP380329CWF
CSB-BP380329CWF
CSB-MP380329CWF
CSB-EP380329CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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