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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia mallei ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP380650BPR
CSB-EP380650BPR
CSB-BP380650BPR
CSB-MP380650BPR
CSB-EP380650BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Histidine--tRNA ligase (hisS) CSB-YP380651BPR
CSB-EP380651BPR
CSB-BP380651BPR
CSB-MP380651BPR
CSB-EP380651BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0133 protein BMASAVP1_A1850 (BMASAVP1_A1850) CSB-YP380652BPR
CSB-EP380652BPR
CSB-BP380652BPR
CSB-MP380652BPR
CSB-EP380652BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L31 type B (rpmE2) CSB-YP380653BPR
CSB-EP380653BPR
CSB-BP380653BPR
CSB-MP380653BPR
CSB-EP380653BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Trigger factor (tig) CSB-YP380654BPR
CSB-EP380654BPR
CSB-BP380654BPR
CSB-MP380654BPR
CSB-EP380654BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Lipid-A-disaccharide synthase (lpxB) CSB-YP380655BPR
CSB-EP380655BPR
CSB-BP380655BPR
CSB-MP380655BPR
CSB-EP380655BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP380656BPR
CSB-EP380656BPR
CSB-BP380656BPR
CSB-MP380656BPR
CSB-EP380656BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP380657BPR
CSB-EP380657BPR
CSB-BP380657BPR
CSB-MP380657BPR
CSB-EP380657BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP380658BPR
CSB-EP380658BPR
CSB-BP380658BPR
CSB-MP380658BPR
CSB-EP380658BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Lysine--tRNA ligase (lysS), partial CSB-YP380659BPR
CSB-EP380659BPR
CSB-BP380659BPR
CSB-MP380659BPR
CSB-EP380659BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Probable Fe (2+)-trafficking protein CSB-YP380660BPR
CSB-EP380660BPR
CSB-BP380660BPR
CSB-MP380660BPR
CSB-EP380660BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Methionine--tRNA ligase (metG), partial CSB-YP380661BPR
CSB-EP380661BPR
CSB-BP380661BPR
CSB-MP380661BPR
CSB-EP380661BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP380662BPR1
CSB-EP380662BPR1
CSB-BP380662BPR1
CSB-MP380662BPR1
CSB-EP380662BPR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei N-succinylarginine dihydrolase (astB) CSB-YP380663BPR
CSB-EP380663BPR
CSB-BP380663BPR
CSB-MP380663BPR
CSB-EP380663BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP380664BPR
CSB-EP380664BPR
CSB-BP380664BPR
CSB-MP380664BPR
CSB-EP380664BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L19 (rplS) CSB-YP380665BPR
CSB-EP380665BPR
CSB-BP380665BPR
CSB-MP380665BPR
CSB-EP380665BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Ribosome maturation factor RimM (rimM) CSB-YP380666BPR
CSB-EP380666BPR
CSB-BP380666BPR
CSB-MP380666BPR
CSB-EP380666BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Cytidylate kinase (cmk) CSB-YP380667BPR
CSB-EP380667BPR
CSB-BP380667BPR
CSB-MP380667BPR
CSB-EP380667BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0758 protein BMASAVP1_A2646 (BMASAVP1_A2646) CSB-YP380668BPR
CSB-EP380668BPR
CSB-BP380668BPR
CSB-MP380668BPR
CSB-EP380668BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP380669BPR
CSB-EP380669BPR
CSB-BP380669BPR
CSB-MP380669BPR
CSB-EP380669BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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