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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus sanguinis tRNA pseudouridine synthase B (truB) CSB-YP386178SUR
CSB-EP386178SUR
CSB-BP386178SUR
CSB-MP386178SUR
CSB-EP386178SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis 30S ribosomal protein S20 (rpsT) CSB-YP386179SUR
CSB-EP386179SUR
CSB-BP386179SUR
CSB-MP386179SUR
CSB-EP386179SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Spermidine/putrescine import ATP-binding protein PotA (potA) CSB-YP386180SUR
CSB-EP386180SUR
CSB-BP386180SUR
CSB-MP386180SUR
CSB-EP386180SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Probable tRNA sulfurtransferase (thiI) CSB-YP386181SUR
CSB-EP386181SUR
CSB-BP386181SUR
CSB-MP386181SUR
CSB-EP386181SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Glutamate 5-kinase (proB) CSB-YP386182SUR
CSB-EP386182SUR
CSB-BP386182SUR
CSB-MP386182SUR
CSB-EP386182SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis 50S ribosomal protein L10 (rplJ) CSB-YP386183SUR
CSB-EP386183SUR
CSB-BP386183SUR
CSB-MP386183SUR
CSB-EP386183SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Dihydrodipicolinate synthase (dapA) CSB-YP386184SUR
CSB-EP386184SUR
CSB-BP386184SUR
CSB-MP386184SUR
CSB-EP386184SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis L-lactate dehydrogenase (ldh) CSB-YP386185SUR
CSB-EP386185SUR
CSB-BP386185SUR
CSB-MP386185SUR
CSB-EP386185SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP386186SUR
CSB-EP386186SUR
CSB-BP386186SUR
CSB-MP386186SUR
CSB-EP386186SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis 50S ribosomal protein L19 (rplS) CSB-YP386187SUR
CSB-EP386187SUR
CSB-BP386187SUR
CSB-MP386187SUR
CSB-EP386187SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Ribosome maturation factor RimM (rimM) CSB-YP386188SUR
CSB-EP386188SUR
CSB-BP386188SUR
CSB-MP386188SUR
CSB-EP386188SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Asparagine--tRNA ligase (asnS) CSB-YP386189SUR
CSB-EP386189SUR
CSB-BP386189SUR
CSB-MP386189SUR
CSB-EP386189SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Histidinol-phosphate aminotransferase (hisC) CSB-YP386190SUR
CSB-EP386190SUR
CSB-BP386190SUR
CSB-MP386190SUR
CSB-EP386190SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis 3-dehydroquinate synthase (aroB) CSB-YP386191SUR
CSB-EP386191SUR
CSB-BP386191SUR
CSB-MP386191SUR
CSB-EP386191SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis DNA ligase (ligA), partial CSB-YP386192SUR
CSB-EP386192SUR
CSB-BP386192SUR
CSB-MP386192SUR
CSB-EP386192SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis 50S ribosomal protein L20 (rplT) CSB-YP386193SUR
CSB-EP386193SUR
CSB-BP386193SUR
CSB-MP386193SUR
CSB-EP386193SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP386194SUR1
CSB-EP386194SUR1
CSB-BP386194SUR1
CSB-MP386194SUR1
CSB-EP386194SUR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis SsrA-binding protein (smpB) CSB-YP386195SUR
CSB-EP386195SUR
CSB-BP386195SUR
CSB-MP386195SUR
CSB-EP386195SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Probable rRNA maturation factor (SSA_1613) CSB-YP386196SUR
CSB-EP386196SUR
CSB-BP386196SUR
CSB-MP386196SUR
CSB-EP386196SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Tagatose-6-phosphate kinase (lacC) CSB-YP386197SUR
CSB-EP386197SUR
CSB-BP386197SUR
CSB-MP386197SUR
CSB-EP386197SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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