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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Clostridium thermocellum Aspartate carbamoyltransferase (pyrB) CSB-YP385177DUT
CSB-EP385177DUT
CSB-BP385177DUT
CSB-MP385177DUT
CSB-EP385177DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP385178DUT
CSB-EP385178DUT
CSB-BP385178DUT
CSB-MP385178DUT
CSB-EP385178DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Ribosome-binding factor A (rbfA) CSB-YP385179DUT
CSB-EP385179DUT
CSB-BP385179DUT
CSB-MP385179DUT
CSB-EP385179DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum MutS2 protein (mutS2), partial CSB-YP385180DUT
CSB-EP385180DUT
CSB-BP385180DUT
CSB-MP385180DUT
CSB-EP385180DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum GTPase Der (der) CSB-YP385181DUT
CSB-EP385181DUT
CSB-BP385181DUT
CSB-MP385181DUT
CSB-EP385181DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP385182DUT
CSB-EP385182DUT
CSB-BP385182DUT
CSB-MP385182DUT
CSB-EP385182DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Bifunctional protein FolD (folD) CSB-YP385183DUT
CSB-EP385183DUT
CSB-BP385183DUT
CSB-MP385183DUT
CSB-EP385183DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 5-methylthioadenosine/S-adenosylhomocysteine deaminase (mtaD) CSB-YP385184DUT
CSB-EP385184DUT
CSB-BP385184DUT
CSB-MP385184DUT
CSB-EP385184DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP385185DUT
CSB-EP385185DUT
CSB-BP385185DUT
CSB-MP385185DUT
CSB-EP385185DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP385186DUT
CSB-EP385186DUT
CSB-BP385186DUT
CSB-MP385186DUT
CSB-EP385186DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Ribosomal protein L11 methyltransferase (prmA) CSB-YP385187DUT
CSB-EP385187DUT
CSB-BP385187DUT
CSB-MP385187DUT
CSB-EP385187DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Chaperone protein DnaK (dnaK), partial CSB-YP385188DUT
CSB-EP385188DUT
CSB-BP385188DUT
CSB-MP385188DUT
CSB-EP385188DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Histidine--tRNA ligase (hisS) CSB-YP385189DUT
CSB-EP385189DUT
CSB-BP385189DUT
CSB-MP385189DUT
CSB-EP385189DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 30S ribosomal protein S21 (rpsU) CSB-YP385190DUT
CSB-EP385190DUT
CSB-BP385190DUT
CSB-MP385190DUT
CSB-EP385190DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Xyloglucanase Xgh74A (xghA), partial CSB-YP385191DUT
CSB-EP385191DUT
CSB-BP385191DUT
CSB-MP385191DUT
CSB-EP385191DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 30S ribosomal protein S9 (rpsI) CSB-YP385192DUT
CSB-EP385192DUT
CSB-BP385192DUT
CSB-MP385192DUT
CSB-EP385192DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP385193DUT
CSB-EP385193DUT
CSB-BP385193DUT
CSB-MP385193DUT
CSB-EP385193DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial CSB-YP385194DUT
CSB-EP385194DUT
CSB-BP385194DUT
CSB-MP385194DUT
CSB-EP385194DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum V-type ATP synthase alpha chain (atpA), partial CSB-YP385195DUT
CSB-EP385195DUT
CSB-BP385195DUT
CSB-MP385195DUT
CSB-EP385195DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum V-type ATP synthase subunit D (atpD) CSB-YP385196DUT
CSB-EP385196DUT
CSB-BP385196DUT
CSB-MP385196DUT
CSB-EP385196DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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