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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia pseudomallei Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP384457BPU
CSB-EP384457BPU
CSB-BP384457BPU
CSB-MP384457BPU
CSB-EP384457BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Recombination protein RecR (recR) CSB-YP384458BPU
CSB-EP384458BPU
CSB-BP384458BPU
CSB-MP384458BPU
CSB-EP384458BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L9 (rplI) CSB-YP384459BPU
CSB-EP384459BPU
CSB-BP384459BPU
CSB-MP384459BPU
CSB-EP384459BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP384460BPU
CSB-EP384460BPU
CSB-BP384460BPU
CSB-MP384460BPU
CSB-EP384460BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP384461BPU
CSB-EP384461BPU
CSB-BP384461BPU
CSB-MP384461BPU
CSB-EP384461BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Lipid-A-disaccharide synthase (lpxB) CSB-YP384462BPU
CSB-EP384462BPU
CSB-BP384462BPU
CSB-MP384462BPU
CSB-EP384462BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP384463BPU
CSB-EP384463BPU
CSB-BP384463BPU
CSB-MP384463BPU
CSB-EP384463BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Uridylate kinase (pyrH) CSB-YP384464BPU
CSB-EP384464BPU
CSB-BP384464BPU
CSB-MP384464BPU
CSB-EP384464BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei [Protein-PII] uridylyltransferase (glnD), partial CSB-YP384465BPU
CSB-EP384465BPU
CSB-BP384465BPU
CSB-MP384465BPU
CSB-EP384465BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Putative regulator of ribonuclease activity (BURPS668_2477) CSB-YP384466BPU
CSB-EP384466BPU
CSB-BP384466BPU
CSB-MP384466BPU
CSB-EP384466BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Cysteine--tRNA ligase (cysS) CSB-YP384467BPU
CSB-EP384467BPU
CSB-BP384467BPU
CSB-MP384467BPU
CSB-EP384467BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Lysine--tRNA ligase (lysS), partial CSB-YP384468BPU
CSB-EP384468BPU
CSB-BP384468BPU
CSB-MP384468BPU
CSB-EP384468BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Imidazolonepropionase (hutI) CSB-YP384469BPU
CSB-EP384469BPU
CSB-BP384469BPU
CSB-MP384469BPU
CSB-EP384469BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP384470BPU
CSB-EP384470BPU
CSB-BP384470BPU
CSB-MP384470BPU
CSB-EP384470BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei tRNA (guanine-N (1)-)-methyltransferase CSB-YP384471BPU
CSB-EP384471BPU
CSB-BP384471BPU
CSB-MP384471BPU
CSB-EP384471BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei D-amino acid dehydrogenase small subunit (dadA) CSB-YP384472BPU
CSB-EP384472BPU
CSB-BP384472BPU
CSB-MP384472BPU
CSB-EP384472BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP384473BPU
CSB-EP384473BPU
CSB-BP384473BPU
CSB-MP384473BPU
CSB-EP384473BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Serine--tRNA ligase (serS) CSB-YP384474BPU
CSB-EP384474BPU
CSB-BP384474BPU
CSB-MP384474BPU
CSB-EP384474BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Protein CrcB homolog (crcB), partial CSB-YP384475BPU1
CSB-EP384475BPU1
CSB-BP384475BPU1
CSB-MP384475BPU1
CSB-EP384475BPU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Urease subunit beta (ureB) CSB-YP384476BPU
CSB-EP384476BPU
CSB-BP384476BPU
CSB-MP384476BPU
CSB-EP384476BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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