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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus Putative pterin-4-alpha-carbinolamine dehydratase (P9301_05181) CSB-YP384618PZE
CSB-EP384618PZE
CSB-BP384618PZE
CSB-MP384618PZE
CSB-EP384618PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein (chlL) CSB-YP384619PZE
CSB-EP384619PZE
CSB-BP384619PZE
CSB-MP384619PZE
CSB-EP384619PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus ATP phosphoribosyltransferase (hisG) CSB-YP384620PZE
CSB-EP384620PZE
CSB-BP384620PZE
CSB-MP384620PZE
CSB-EP384620PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Homoserine kinase (thrB) CSB-YP384621PZE
CSB-EP384621PZE
CSB-BP384621PZE
CSB-MP384621PZE
CSB-EP384621PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Bifunctional protein GlmU (glmU) CSB-YP384622PZE
CSB-EP384622PZE
CSB-BP384622PZE
CSB-MP384622PZE
CSB-EP384622PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 33 kDa chaperonin (hslO) CSB-YP384623PZE
CSB-EP384623PZE
CSB-BP384623PZE
CSB-MP384623PZE
CSB-EP384623PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S2 (rpsB) CSB-YP384624PZE
CSB-EP384624PZE
CSB-BP384624PZE
CSB-MP384624PZE
CSB-EP384624PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome c biogenesis protein CcsA (ccsA), partial CSB-YP384625PZE
CSB-EP384625PZE
CSB-BP384625PZE
CSB-MP384625PZE
CSB-EP384625PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus D-fructose 1,6-bisphosphatase class 2/sedoheptulose 1,7-bisphosphatase (P9301_08271) CSB-YP384626PZE
CSB-EP384626PZE
CSB-BP384626PZE
CSB-MP384626PZE
CSB-EP384626PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Urease accessory protein UreF (ureF) CSB-YP384627PZE
CSB-EP384627PZE
CSB-BP384627PZE
CSB-MP384627PZE
CSB-EP384627PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Urease subunit beta (ureB) CSB-YP384628PZE
CSB-EP384628PZE
CSB-BP384628PZE
CSB-MP384628PZE
CSB-EP384628PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP384629PZE
CSB-EP384629PZE
CSB-BP384629PZE
CSB-MP384629PZE
CSB-EP384629PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II reaction center Psb28 protein (psb28) CSB-YP384630PZE
CSB-EP384630PZE
CSB-BP384630PZE
CSB-MP384630PZE
CSB-EP384630PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Leucine--tRNA ligase (leuS), partial CSB-YP384631PZE
CSB-EP384631PZE
CSB-BP384631PZE
CSB-MP384631PZE
CSB-EP384631PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L32 (rpmF) CSB-YP384632PZE
CSB-EP384632PZE
CSB-BP384632PZE
CSB-MP384632PZE
CSB-EP384632PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S21 (rpsU) CSB-YP384633PZE
CSB-EP384633PZE
CSB-BP384633PZE
CSB-MP384633PZE
CSB-EP384633PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP384634PZE
CSB-EP384634PZE
CSB-BP384634PZE
CSB-MP384634PZE
CSB-EP384634PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Adaptive-response sensory-kinase sasA (sasA) CSB-YP384635PZE
CSB-EP384635PZE
CSB-BP384635PZE
CSB-MP384635PZE
CSB-EP384635PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cysteine--tRNA ligase (cysS) CSB-YP384636PZE
CSB-EP384636PZE
CSB-BP384636PZE
CSB-MP384636PZE
CSB-EP384636PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus PKHD-type hydroxylase P9301_13621 (P9301_13621) CSB-YP384637PZE
CSB-EP384637PZE
CSB-BP384637PZE
CSB-MP384637PZE
CSB-EP384637PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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