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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Clostridium thermocellum 50S ribosomal protein L16 (rplP) CSB-YP386418DUT
CSB-EP386418DUT
CSB-BP386418DUT
CSB-MP386418DUT
CSB-EP386418DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L24 (rplX) CSB-YP386419DUT
CSB-EP386419DUT
CSB-BP386419DUT
CSB-MP386419DUT
CSB-EP386419DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L18 (rplR) CSB-YP386420DUT
CSB-EP386420DUT
CSB-BP386420DUT
CSB-MP386420DUT
CSB-EP386420DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Translation initiation factor IF-1 (infA) CSB-YP386421DUT
CSB-EP386421DUT
CSB-BP386421DUT
CSB-MP386421DUT
CSB-EP386421DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L17 (rplQ) CSB-YP386422DUT
CSB-EP386422DUT
CSB-BP386422DUT
CSB-MP386422DUT
CSB-EP386422DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Proline--tRNA ligase (proS), partial CSB-YP386423DUT
CSB-EP386423DUT
CSB-BP386423DUT
CSB-MP386423DUT
CSB-EP386423DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Adenylosuccinate synthetase (purA) CSB-YP386424DUT
CSB-EP386424DUT
CSB-BP386424DUT
CSB-MP386424DUT
CSB-EP386424DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP386425DUT
CSB-EP386425DUT
CSB-BP386425DUT
CSB-MP386425DUT
CSB-EP386425DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Chromatin protein Cren7 (creN7) CSB-YP386426SUP
CSB-EP386426SUP
CSB-BP386426SUP
CSB-MP386426SUP
CSB-EP386426SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus D-tyrosyl-tRNA (Tyr) deacylase CSB-YP386427SUP
CSB-EP386427SUP
CSB-BP386427SUP
CSB-MP386427SUP
CSB-EP386427SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Aspartate carbamoyltransferase (pyrB) CSB-YP386428SUP
CSB-EP386428SUP
CSB-BP386428SUP
CSB-MP386428SUP
CSB-EP386428SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus RNA-binding protein AU-1 (aubA) CSB-YP386429SUP
CSB-EP386429SUP
CSB-BP386429SUP
CSB-MP386429SUP
CSB-EP386429SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Ribosome biogenesis protein Nop10 (Smar_0644) CSB-YP386430SUP
CSB-EP386430SUP
CSB-BP386430SUP
CSB-MP386430SUP
CSB-EP386430SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Peptidyl-tRNA hydrolase (pth) CSB-YP386431SUP
CSB-EP386431SUP
CSB-BP386431SUP
CSB-MP386431SUP
CSB-EP386431SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Maf-like protein Smar_0734 (Smar_0734) CSB-YP386432SUP
CSB-EP386432SUP
CSB-BP386432SUP
CSB-MP386432SUP
CSB-EP386432SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Elongation factor 1-alpha (tuf) CSB-YP386433SUP
CSB-EP386433SUP
CSB-BP386433SUP
CSB-MP386433SUP
CSB-EP386433SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus 30S ribosomal protein S11P (rps11p) CSB-YP386434SUP
CSB-EP386434SUP
CSB-BP386434SUP
CSB-MP386434SUP
CSB-EP386434SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus 50S ribosomal protein L7Ae (rpl7Ae) CSB-YP386435SUP
CSB-EP386435SUP
CSB-BP386435SUP
CSB-MP386435SUP
CSB-EP386435SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus UPF0218 protein Smar_0835 (Smar_0835) CSB-YP386436SUP
CSB-EP386436SUP
CSB-BP386436SUP
CSB-MP386436SUP
CSB-EP386436SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Probable tRNA threonylcarbamoyladenosine biosynthesis protein KAE1 homolog (Smar_0838) CSB-YP386437SUP
CSB-EP386437SUP
CSB-BP386437SUP
CSB-MP386437SUP
CSB-EP386437SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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