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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Prochlorococcus marinus Phycoerythrobilin:ferredoxin oxidoreductase (pebB) CSB-YP384657PZE
CSB-EP384657PZE
CSB-BP384657PZE
CSB-MP384657PZE
CSB-EP384657PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem I iron-sulfur center (psaC) CSB-YP384658PZE
CSB-EP384658PZE
CSB-BP384658PZE
CSB-MP384658PZE
CSB-EP384658PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP384659PZE
CSB-EP384659PZE
CSB-BP384659PZE
CSB-MP384659PZE
CSB-EP384659PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP384660PZE
CSB-EP384660PZE
CSB-BP384660PZE
CSB-MP384660PZE
CSB-EP384660PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L20 (rplT) CSB-YP384661PZE
CSB-EP384661PZE
CSB-BP384661PZE
CSB-MP384661PZE
CSB-EP384661PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP384662PZE
CSB-EP384662PZE
CSB-BP384662PZE
CSB-MP384662PZE
CSB-EP384662PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Aspartate--tRNA ligase (aspS), partial CSB-YP384663PZE
CSB-EP384663PZE
CSB-BP384663PZE
CSB-MP384663PZE
CSB-EP384663PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides UPF0246 protein Rsph17029_0026 (Rsph17029_0026) CSB-YP384664RIJ
CSB-EP384664RIJ
CSB-BP384664RIJ
CSB-MP384664RIJ
CSB-EP384664RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP384665RIJ
CSB-EP384665RIJ
CSB-BP384665RIJ
CSB-MP384665RIJ
CSB-EP384665RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP384666RIJ1
CSB-EP384666RIJ1
CSB-BP384666RIJ1
CSB-MP384666RIJ1
CSB-EP384666RIJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP384667RIJ
CSB-EP384667RIJ
CSB-BP384667RIJ
CSB-MP384667RIJ
CSB-EP384667RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides DNA repair protein recO (recO) CSB-YP384668RIJ
CSB-EP384668RIJ
CSB-BP384668RIJ
CSB-MP384668RIJ
CSB-EP384668RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides UPF0042 nucleotide-binding protein Rsph17029_0317 (Rsph17029_0317) CSB-YP384669RIJ
CSB-EP384669RIJ
CSB-BP384669RIJ
CSB-MP384669RIJ
CSB-EP384669RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L1 (rplA1) CSB-YP384670RIJ
CSB-EP384670RIJ
CSB-BP384670RIJ
CSB-MP384670RIJ
CSB-EP384670RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L4 (rplD) CSB-YP384671RIJ
CSB-EP384671RIJ
CSB-BP384671RIJ
CSB-MP384671RIJ
CSB-EP384671RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 30S ribosomal protein S3 (rpsC) CSB-YP384672RIJ
CSB-EP384672RIJ
CSB-BP384672RIJ
CSB-MP384672RIJ
CSB-EP384672RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 30S ribosomal protein S5 (rpsE) CSB-YP384673RIJ
CSB-EP384673RIJ
CSB-BP384673RIJ
CSB-MP384673RIJ
CSB-EP384673RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 30S ribosomal protein S13 (rpsM) CSB-YP384674RIJ
CSB-EP384674RIJ
CSB-BP384674RIJ
CSB-MP384674RIJ
CSB-EP384674RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Protein CrcB homolog (crcB), partial CSB-YP384675RIJ1
CSB-EP384675RIJ1
CSB-BP384675RIJ1
CSB-MP384675RIJ1
CSB-EP384675RIJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Pantothenate synthetase (panC) CSB-YP384676RIJ
CSB-EP384676RIJ
CSB-BP384676RIJ
CSB-MP384676RIJ
CSB-EP384676RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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