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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Clostridium thermocellum Probable RNA 2'-phosphotransferase (kptA) CSB-YP385217DUT
CSB-EP385217DUT
CSB-BP385217DUT
CSB-MP385217DUT
CSB-EP385217DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum LL-diaminopimelate aminotransferase (dapL) CSB-YP385218DUT
CSB-EP385218DUT
CSB-BP385218DUT
CSB-MP385218DUT
CSB-EP385218DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Leucine--tRNA ligase (leuS), partial CSB-YP385219SUP
CSB-EP385219SUP
CSB-BP385219SUP
CSB-MP385219SUP
CSB-EP385219SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus UPF0278 protein Smar_0256 (Smar_0256) CSB-YP385220SUP
CSB-EP385220SUP
CSB-BP385220SUP
CSB-MP385220SUP
CSB-EP385220SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Adenine deaminase (ade), partial CSB-YP385221SUP
CSB-EP385221SUP
CSB-BP385221SUP
CSB-MP385221SUP
CSB-EP385221SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Arginine decarboxylase proenzyme (Smar_0500) CSB-YP385222SUP
CSB-EP385222SUP
CSB-BP385222SUP
CSB-MP385222SUP
CSB-EP385222SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Cysteine--tRNA ligase (cysS) CSB-YP385223SUP
CSB-EP385223SUP
CSB-BP385223SUP
CSB-MP385223SUP
CSB-EP385223SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus UPF0290 protein Smar_0617 (Smar_0617), partial CSB-YP385224SUP1
CSB-EP385224SUP1
CSB-BP385224SUP1
CSB-MP385224SUP1
CSB-EP385224SUP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Probable DNA primase small subunit (priA) CSB-YP385225SUP
CSB-EP385225SUP
CSB-BP385225SUP
CSB-MP385225SUP
CSB-EP385225SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP385226SUP
CSB-EP385226SUP
CSB-BP385226SUP
CSB-MP385226SUP
CSB-EP385226SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus 50S ribosomal protein L21e (rpl21e) CSB-YP385227SUP
CSB-EP385227SUP
CSB-BP385227SUP
CSB-MP385227SUP
CSB-EP385227SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus 30S ribosomal protein S13P (rps13p) CSB-YP385228SUP
CSB-EP385228SUP
CSB-BP385228SUP
CSB-MP385228SUP
CSB-EP385228SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus DNA-directed RNA polymerase subunit N (rpoN) CSB-YP385229SUP
CSB-EP385229SUP
CSB-BP385229SUP
CSB-MP385229SUP
CSB-EP385229SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus 50S ribosomal protein L24e (rpl24e) CSB-YP385230SUP
CSB-EP385230SUP
CSB-BP385230SUP
CSB-MP385230SUP
CSB-EP385230SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Probable translation initiation factor IF-2 (infB), partial CSB-YP385231SUP
CSB-EP385231SUP
CSB-BP385231SUP
CSB-MP385231SUP
CSB-EP385231SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus UPF0173 metal-dependent hydrolase Smar_0891 (Smar_0891) CSB-YP385232SUP
CSB-EP385232SUP
CSB-BP385232SUP
CSB-MP385232SUP
CSB-EP385232SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus RNA 3'-terminal phosphate cyclase (rtcA) CSB-YP385233SUP
CSB-EP385233SUP
CSB-BP385233SUP
CSB-MP385233SUP
CSB-EP385233SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Glycerol-1-phosphate dehydrogenase [NAD (P)+] CSB-YP385234SUP
CSB-EP385234SUP
CSB-BP385234SUP
CSB-MP385234SUP
CSB-EP385234SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Geranylgeranylglyceryl phosphate synthase (Smar_0993) CSB-YP385235SUP
CSB-EP385235SUP
CSB-BP385235SUP
CSB-MP385235SUP
CSB-EP385235SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus 50S ribosomal protein L23P (rpl23p) CSB-YP385236SUP
CSB-EP385236SUP
CSB-BP385236SUP
CSB-MP385236SUP
CSB-EP385236SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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