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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L20 (rplT) CSB-YP384677RIJ
CSB-EP384677RIJ
CSB-BP384677RIJ
CSB-MP384677RIJ
CSB-EP384677RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Transcription elongation factor GreA (greA) CSB-YP384678RIJ
CSB-EP384678RIJ
CSB-BP384678RIJ
CSB-MP384678RIJ
CSB-EP384678RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP384679RIJ
CSB-EP384679RIJ
CSB-BP384679RIJ
CSB-MP384679RIJ
CSB-EP384679RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP384680BPP
CSB-EP384680BPP
CSB-BP384680BPP
CSB-MP384680BPP
CSB-EP384680BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei HPr kinase/phosphorylase (hprK) CSB-YP384681BPP
CSB-EP384681BPP
CSB-BP384681BPP
CSB-MP384681BPP
CSB-EP384681BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP384682BPP
CSB-EP384682BPP
CSB-BP384682BPP
CSB-MP384682BPP
CSB-EP384682BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0102 protein BMA10229_A1742 (BMA10229_A1742) CSB-YP384683BPP
CSB-EP384683BPP
CSB-BP384683BPP
CSB-MP384683BPP
CSB-EP384683BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP384684BPP
CSB-EP384684BPP
CSB-BP384684BPP
CSB-MP384684BPP
CSB-EP384684BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Sec-independent protein translocase protein TatA (tatA), partial CSB-YP384685BPP
CSB-EP384685BPP
CSB-BP384685BPP
CSB-MP384685BPP
CSB-EP384685BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L10 (rplJ) CSB-YP384686BPP
CSB-EP384686BPP
CSB-BP384686BPP
CSB-MP384686BPP
CSB-EP384686BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 30S ribosomal protein S7 (rpsG) CSB-YP384687BPP
CSB-EP384687BPP
CSB-BP384687BPP
CSB-MP384687BPP
CSB-EP384687BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L3 (rplC) CSB-YP384688BPP
CSB-EP384688BPP
CSB-BP384688BPP
CSB-MP384688BPP
CSB-EP384688BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 30S ribosomal protein S19 (rpsS) CSB-YP384689BPP
CSB-EP384689BPP
CSB-BP384689BPP
CSB-MP384689BPP
CSB-EP384689BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 30S ribosomal protein S17 (rpsQ) CSB-YP384690BPP
CSB-EP384690BPP
CSB-BP384690BPP
CSB-MP384690BPP
CSB-EP384690BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 30S ribosomal protein S14 (rpsN) CSB-YP384691BPP
CSB-EP384691BPP
CSB-BP384691BPP
CSB-MP384691BPP
CSB-EP384691BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L30 (rpmD) CSB-YP384692BPP
CSB-EP384692BPP
CSB-BP384692BPP
CSB-MP384692BPP
CSB-EP384692BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 30S ribosomal protein S13 (rpsM) CSB-YP384693BPP
CSB-EP384693BPP
CSB-BP384693BPP
CSB-MP384693BPP
CSB-EP384693BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L17 (rplQ) CSB-YP384694BPP
CSB-EP384694BPP
CSB-BP384694BPP
CSB-MP384694BPP
CSB-EP384694BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 8-amino-7-oxononanoate synthase (bioF) CSB-YP384695BPP
CSB-EP384695BPP
CSB-BP384695BPP
CSB-MP384695BPP
CSB-EP384695BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP384696BPP
CSB-EP384696BPP
CSB-BP384696BPP
CSB-MP384696BPP
CSB-EP384696BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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