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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Oryza sativa subsp. indica Cation transporter HKT8 (HKT8), partial CSB-YP384857OFF
CSB-EP384857OFF
CSB-BP384857OFF
CSB-MP384857OFF
CSB-EP384857OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Probable histone H2A.5 (OsI_002060) CSB-YP384858OFF
CSB-EP384858OFF
CSB-BP384858OFF
CSB-MP384858OFF
CSB-EP384858OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Bidirectional sugar transporter SWEET6a (SWEET6A), partial CSB-YP384859OFF1
CSB-EP384859OFF1
CSB-BP384859OFF1
CSB-MP384859OFF1
CSB-EP384859OFF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Phospholipase A1-II 1 (OsI_03083) CSB-YP384860OFF
CSB-EP384860OFF
CSB-BP384860OFF
CSB-MP384860OFF
CSB-EP384860OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Transcription factor PCF7 (PCF7) CSB-YP384861OFF
CSB-EP384861OFF
CSB-BP384861OFF
CSB-MP384861OFF
CSB-EP384861OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Histone H2B.11 (H2B.11) CSB-YP384862OFF
CSB-EP384862OFF
CSB-BP384862OFF
CSB-MP384862OFF
CSB-EP384862OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica 60S ribosomal protein L5-1 (RPL5A) CSB-YP384863OFF
CSB-EP384863OFF
CSB-BP384863OFF
CSB-MP384863OFF
CSB-EP384863OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Acyl-[acyl-carrier-protein] desaturase 2, chloroplastic (SSI2) CSB-YP384864OFF
CSB-EP384864OFF
CSB-BP384864OFF
CSB-MP384864OFF
CSB-EP384864OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Probable esterase PIR7A (PIR7A) CSB-YP384865OFF
CSB-EP384865OFF
CSB-BP384865OFF
CSB-MP384865OFF
CSB-EP384865OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Outer envelope protein 80, chloroplastic (OEP80), partial CSB-YP384866OFF
CSB-EP384866OFF
CSB-BP384866OFF
CSB-MP384866OFF
CSB-EP384866OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Bidirectional sugar transporter SWEET4 (SWEET4), partial CSB-YP384867OFF1
CSB-EP384867OFF1
CSB-BP384867OFF1
CSB-MP384867OFF1
CSB-EP384867OFF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Homeobox-leucine zipper protein HOX7 (HOX7) CSB-YP384868OFF
CSB-EP384868OFF
CSB-BP384868OFF
CSB-MP384868OFF
CSB-EP384868OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Protein YABBY 4 (YAB4) CSB-YP384869OFF
CSB-EP384869OFF
CSB-BP384869OFF
CSB-MP384869OFF
CSB-EP384869OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Soluble inorganic pyrophosphatase (IPP) CSB-YP384870OFF
CSB-EP384870OFF
CSB-BP384870OFF
CSB-MP384870OFF
CSB-EP384870OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Uncharacterized protein OsI_009113 (OsI_009113) CSB-YP384871OFF
CSB-EP384871OFF
CSB-BP384871OFF
CSB-MP384871OFF
CSB-EP384871OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Auxin-responsive protein IAA10 (IAA10) CSB-YP384872OFF
CSB-EP384872OFF
CSB-BP384872OFF
CSB-MP384872OFF
CSB-EP384872OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Protein-ribulosamine 3-kinase, chloroplastic (OsI_09747) CSB-YP384873OFF
CSB-EP384873OFF
CSB-BP384873OFF
CSB-MP384873OFF
CSB-EP384873OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Adenylosuccinate synthetase, chloroplastic (PURA) CSB-YP384874OFF
CSB-EP384874OFF
CSB-BP384874OFF
CSB-MP384874OFF
CSB-EP384874OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Histone H2B.1 (OsI_010709) CSB-YP384875OFF
CSB-EP384875OFF
CSB-BP384875OFF
CSB-MP384875OFF
CSB-EP384875OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Mitogen-activated protein kinase 5 (MPK5) CSB-YP384876OFF
CSB-EP384876OFF
CSB-BP384876OFF
CSB-MP384876OFF
CSB-EP384876OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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