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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Oryza sativa subsp. indica Nicotianamine synthase 1 (NAS1) CSB-YP384877OFF
CSB-EP384877OFF
CSB-BP384877OFF
CSB-MP384877OFF
CSB-EP384877OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica WUSCHEL-related homeobox 6 (WOX6) CSB-YP384878OFF
CSB-EP384878OFF
CSB-BP384878OFF
CSB-MP384878OFF
CSB-EP384878OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica SPX domain-containing protein 5 (SPX5) CSB-YP384879OFF
CSB-EP384879OFF
CSB-BP384879OFF
CSB-MP384879OFF
CSB-EP384879OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Chlorophyll a-b binding protein, chloroplastic (OsI_012078), partial CSB-YP384880OFF1
CSB-EP384880OFF1
CSB-BP384880OFF1
CSB-MP384880OFF1
CSB-EP384880OFF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Probable E3 ubiquitin-protein ligase BAH1-like 1 (OsI_12825) CSB-YP384881OFF
CSB-EP384881OFF
CSB-BP384881OFF
CSB-MP384881OFF
CSB-EP384881OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Probable histone H2AXa (OsI_012923) CSB-YP384882OFF
CSB-EP384882OFF
CSB-BP384882OFF
CSB-MP384882OFF
CSB-EP384882OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica 10 kDa prolamin (OsI_013205) CSB-YP384883OFF
CSB-BP384883OFF
CSB-MP384883OFF
CSB-EP384883OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Transcription factor PCF6 (PCF6) CSB-YP384884OFF
CSB-EP384884OFF
CSB-BP384884OFF
CSB-MP384884OFF
CSB-EP384884OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Chalcone--flavonone isomerase (CHI) CSB-YP384885OFF
CSB-EP384885OFF
CSB-BP384885OFF
CSB-MP384885OFF
CSB-EP384885OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Probable cellulose synthase A catalytic subunit 5 [UDP-forming] (CESA5), partial CSB-YP384886OFF
CSB-EP384886OFF
CSB-BP384886OFF
CSB-MP384886OFF
CSB-EP384886OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Probable aldo-keto reductase 3 (H0813E03.4, OsI_15393) CSB-YP384887OFF
CSB-EP384887OFF
CSB-BP384887OFF
CSB-MP384887OFF
CSB-EP384887OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica PHD finger protein ALFIN-LIKE 4 (OsI_16049) CSB-YP384888OFF
CSB-EP384888OFF
CSB-BP384888OFF
CSB-MP384888OFF
CSB-EP384888OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Peroxygenase (PXG) CSB-YP384889OFF
CSB-EP384889OFF
CSB-BP384889OFF
CSB-MP384889OFF
CSB-EP384889OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Dehydration-responsive element-binding protein 1E (DREB1E) CSB-YP384890OFF
CSB-EP384890OFF
CSB-BP384890OFF
CSB-MP384890OFF
CSB-EP384890OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Probable protein arginine N-methyltransferase 6.1 (PRMT6.1) CSB-YP384891OFF
CSB-EP384891OFF
CSB-BP384891OFF
CSB-MP384891OFF
CSB-EP384891OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Metallothionein-like protein 2C (MT2C) CSB-YP384892OFF
CSB-EP384892OFF
CSB-BP384892OFF
CSB-MP384892OFF
CSB-EP384892OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica S-adenosylmethionine synthase 1 (SAM1) CSB-YP384893OFF
CSB-EP384893OFF
CSB-BP384893OFF
CSB-MP384893OFF
CSB-EP384893OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica 60S ribosomal protein L10-2 (SG12) CSB-YP384894OFF
CSB-EP384894OFF
CSB-BP384894OFF
CSB-MP384894OFF
CSB-EP384894OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Ferrochelatase-2, chloroplastic (HEMH), partial CSB-YP384895OFF
CSB-EP384895OFF
CSB-BP384895OFF
CSB-MP384895OFF
CSB-EP384895OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica PHD finger protein ALFIN-LIKE 5 (OsI_19996) CSB-YP384896OFF
CSB-EP384896OFF
CSB-BP384896OFF
CSB-MP384896OFF
CSB-EP384896OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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