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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Oryza sativa subsp. indica Probable monogalactosyldiacylglycerol synthase 2, chloroplastic (MGD2) CSB-YP384917OFF
CSB-EP384917OFF
CSB-BP384917OFF
CSB-MP384917OFF
CSB-EP384917OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Probable histone H2A.3 (OsI_028288) CSB-YP384918OFF
CSB-EP384918OFF
CSB-BP384918OFF
CSB-MP384918OFF
CSB-EP384918OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica GDT1-like protein 4 (OsI_29993), partial CSB-YP384919OFF1
CSB-EP384919OFF1
CSB-BP384919OFF1
CSB-MP384919OFF1
CSB-EP384919OFF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Proteasome subunit alpha type-7-A (OsI_029135) CSB-YP384920OFF
CSB-EP384920OFF
CSB-BP384920OFF
CSB-MP384920OFF
CSB-EP384920OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Probable protein arginine N-methyltransferase 1 (PRMT1) CSB-YP384921OFF
CSB-EP384921OFF
CSB-BP384921OFF
CSB-MP384921OFF
CSB-EP384921OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Homeobox-leucine zipper protein HOX25 (HOX25) CSB-YP384922OFF
CSB-EP384922OFF
CSB-BP384922OFF
CSB-MP384922OFF
CSB-EP384922OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Cellulose synthase A catalytic subunit 9 [UDP-forming] (CESA9), partial CSB-YP384923OFF
CSB-EP384923OFF
CSB-BP384923OFF
CSB-MP384923OFF
CSB-EP384923OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Zinc finger A20 and AN1 domain-containing stress-associated protein 1 (SAP1) CSB-YP384924OFF
CSB-EP384924OFF
CSB-BP384924OFF
CSB-MP384924OFF
CSB-EP384924OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Uncharacterized protein OsI_031781 (OsI_031781) CSB-YP384925OFF
CSB-EP384925OFF
CSB-BP384925OFF
CSB-MP384925OFF
CSB-EP384925OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 1 (ARD1) CSB-YP384926OFF
CSB-EP384926OFF
CSB-BP384926OFF
CSB-MP384926OFF
CSB-EP384926OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Glycine cleavage system H protein, mitochondrial (GDCSH) CSB-YP384927OFF
CSB-EP384927OFF
CSB-BP384927OFF
CSB-MP384927OFF
CSB-EP384927OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica E3 ubiquitin-protein ligase BRE1-like 2 (BRE1B), partial CSB-YP384928OFF
CSB-EP384928OFF
CSB-BP384928OFF
CSB-MP384928OFF
CSB-EP384928OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Protein SCARECROW 1 (SCR1), partial CSB-YP384929OFF
CSB-EP384929OFF
CSB-BP384929OFF
CSB-MP384929OFF
CSB-EP384929OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica 60S ribosomal protein L10-1 (SC34) CSB-YP384930OFF
CSB-EP384930OFF
CSB-BP384930OFF
CSB-MP384930OFF
CSB-EP384930OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Dehydrin Rab16B (RAB16B) CSB-YP384931OFF
CSB-EP384931OFF
CSB-BP384931OFF
CSB-MP384931OFF
CSB-EP384931OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Chalcone synthase 1 (CHS1) CSB-YP384932OFF
CSB-EP384932OFF
CSB-BP384932OFF
CSB-MP384932OFF
CSB-EP384932OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Metallothionein-like protein 1A (ERR26) CSB-YP384933OFF
CSB-EP384933OFF
CSB-BP384933OFF
CSB-MP384933OFF
CSB-EP384933OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Non-specific lipid-transfer protein 1 (LTP) CSB-YP384934OFF
CSB-EP384934OFF
CSB-BP384934OFF
CSB-MP384934OFF
CSB-EP384934OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Bidirectional sugar transporter SWEET7c (SWEET7C), partial CSB-YP384935OFF1
CSB-EP384935OFF1
CSB-BP384935OFF1
CSB-MP384935OFF1
CSB-EP384935OFF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Putative glycine-rich cell wall structural protein 1 (GRP-1) CSB-YP384936OFF
CSB-EP384936OFF
CSB-BP384936OFF
CSB-MP384936OFF
CSB-EP384936OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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