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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bat coronavirus HKU5 Envelope small membrane protein (E), partial CSB-YP385257BOU
CSB-EP385257BOU
CSB-BP385257BOU
CSB-MP385257BOU
CSB-EP385257BOU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bat coronavirus HKU9 Spike glycoprotein (S), partial CSB-YP385258BOV
CSB-MP385258BOV
CSB-EP385258BOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bat coronavirus HKU9 Nucleoprotein (N) CSB-YP385259BOV
CSB-EP385259BOV
CSB-MP385259BOV
CSB-EP385259BOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mitogen-activated protein kinase HOG1 (HOG1) CSB-YP385260WBD
CSB-EP385260WBD
CSB-BP385260WBD
CSB-MP385260WBD
CSB-EP385260WBD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mustela putorius furo Interleukin-6 (IL6) CSB-YP385261MOH
CSB-EP385261MOH
CSB-BP385261MOH
CSB-MP385261MOH
CSB-EP385261MOH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nelumbo nucifera Defensin-like protein CSB-YP385262NEY
CSB-EP385262NEY
CSB-BP385262NEY
CSB-MP385262NEY
CSB-EP385262NEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Ubiquitin-related modifier 1 (URM1) CSB-YP385263SXM
CSB-EP385263SXM
CSB-BP385263SXM
CSB-MP385263SXM
CSB-EP385263SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Nuclear protein localization protein 4 (NPL4), partial CSB-YP385264SXM
CSB-EP385264SXM
CSB-BP385264SXM
CSB-MP385264SXM
CSB-EP385264SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Protein TIF31 homolog (TIF31), partial CSB-YP385265SXM
CSB-EP385265SXM
CSB-BP385265SXM
CSB-MP385265SXM
CSB-EP385265SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis U3 small nucleolar RNA-associated protein 10 (UTP10), partial CSB-YP385266SXM
CSB-EP385266SXM
CSB-BP385266SXM
CSB-MP385266SXM
CSB-EP385266SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Probable kynurenine formamidase (BNA7) CSB-YP385267SXM
CSB-EP385267SXM
CSB-BP385267SXM
CSB-MP385267SXM
CSB-EP385267SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Elongation factor G, mitochondrial (MEF1), partial CSB-YP385268SXM
CSB-EP385268SXM
CSB-BP385268SXM
CSB-MP385268SXM
CSB-EP385268SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geranyl diphosphate 2-C-methyltransferase (SAML0358) CSB-YP385269SUV
CSB-EP385269SUV
CSB-BP385269SUV
CSB-MP385269SUV
CSB-EP385269SUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Death-associated protein-like 1-A (dapl1-a) CSB-YP385270XBE
CSB-EP385270XBE
CSB-BP385270XBE
CSB-MP385270XBE
CSB-EP385270XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Death-associated protein-like 1-B (dapl1-b) CSB-YP385271XBE
CSB-EP385271XBE
CSB-BP385271XBE
CSB-MP385271XBE
CSB-EP385271XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Uncharacterized protein C9orf102 homolog, partial CSB-YP385272BO
CSB-EP385272BO
CSB-BP385272BO
CSB-MP385272BO
CSB-EP385272BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine UPF0364 protein C6orf211 homolog CSB-YP385273BO
CSB-EP385273BO
CSB-BP385273BO
CSB-MP385273BO
CSB-EP385273BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Uncharacterized protein C2orf47 homolog, mitochondrial CSB-YP385274BO
CSB-EP385274BO
CSB-BP385274BO
CSB-MP385274BO
CSB-EP385274BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis MIF4G domain-containing protein A (mif4gd-a) CSB-YP385275XBE
CSB-EP385275XBE
CSB-BP385275XBE
CSB-MP385275XBE
CSB-EP385275XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Gamma-glutamylaminecyclotransferase B (zgc:162208) CSB-YP385276DIL
CSB-EP385276DIL
CSB-BP385276DIL
CSB-MP385276DIL
CSB-EP385276DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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