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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Methylibium petroleiphilum Putative phosphotransferase Mpe_A1520 (Mpe_A1520) CSB-YP387098MNX
CSB-EP387098MNX
CSB-BP387098MNX
CSB-MP387098MNX
CSB-EP387098MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP387099MNX
CSB-EP387099MNX
CSB-BP387099MNX
CSB-MP387099MNX
CSB-EP387099MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP387100MNX
CSB-EP387100MNX
CSB-BP387100MNX
CSB-MP387100MNX
CSB-EP387100MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum DNA ligase (ligA), partial CSB-YP387101MNX
CSB-EP387101MNX
CSB-BP387101MNX
CSB-MP387101MNX
CSB-EP387101MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 50S ribosomal protein L35 (rpmI) CSB-YP387102MNX
CSB-EP387102MNX
CSB-BP387102MNX
CSB-MP387102MNX
CSB-EP387102MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum GTPase Der (der) CSB-YP387103MNX
CSB-EP387103MNX
CSB-BP387103MNX
CSB-MP387103MNX
CSB-EP387103MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 2-isopropylmalate synthase (leuA), partial CSB-YP387104MNX
CSB-EP387104MNX
CSB-BP387104MNX
CSB-MP387104MNX
CSB-EP387104MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Dihydroorotate dehydrogenase (quinone) CSB-YP387105MNX
CSB-EP387105MNX
CSB-BP387105MNX
CSB-MP387105MNX
CSB-EP387105MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Tryptophan synthase beta chain (trpB) CSB-YP387106MNX
CSB-EP387106MNX
CSB-BP387106MNX
CSB-MP387106MNX
CSB-EP387106MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP387107MNX
CSB-EP387107MNX
CSB-BP387107MNX
CSB-MP387107MNX
CSB-EP387107MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 4-hydroxy-2-oxovalerate aldolase 1 (Mpe_A2266) CSB-YP387108MNX
CSB-EP387108MNX
CSB-BP387108MNX
CSB-MP387108MNX
CSB-EP387108MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Alanine--tRNA ligase (alaS), partial CSB-YP387109MNX
CSB-EP387109MNX
CSB-BP387109MNX
CSB-MP387109MNX
CSB-EP387109MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum UPF0434 protein Mpe_A2486 (Mpe_A2486) CSB-YP387110MNX
CSB-EP387110MNX
CSB-BP387110MNX
CSB-MP387110MNX
CSB-EP387110MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Adenylate kinase (adk) CSB-YP387111MNX
CSB-EP387111MNX
CSB-BP387111MNX
CSB-MP387111MNX
CSB-EP387111MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Potassium-transporting ATPase C chain (kdpC), partial CSB-YP387112MNX
CSB-EP387112MNX
CSB-BP387112MNX
CSB-MP387112MNX
CSB-EP387112MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Dihydrodipicolinate synthase (dapA) CSB-YP387113MNX
CSB-EP387113MNX
CSB-BP387113MNX
CSB-MP387113MNX
CSB-EP387113MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum UPF0758 protein Mpe_A2695 (Mpe_A2695) CSB-YP387114MNX
CSB-EP387114MNX
CSB-BP387114MNX
CSB-MP387114MNX
CSB-EP387114MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP387115MNX
CSB-EP387115MNX
CSB-BP387115MNX
CSB-MP387115MNX
CSB-EP387115MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Fructose-1,6-bisphosphatase class 1 3 (fbp3) CSB-YP387116MNX
CSB-EP387116MNX
CSB-BP387116MNX
CSB-MP387116MNX
CSB-EP387116MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum tRNA (guanine-N (7)-)-methyltransferase CSB-YP387117MNX
CSB-EP387117MNX
CSB-BP387117MNX
CSB-MP387117MNX
CSB-EP387117MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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