Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia mallei Urease accessory protein UreF (ureF) CSB-YP387779BPQ
CSB-EP387779BPQ
CSB-BP387779BPQ
CSB-MP387779BPQ
CSB-EP387779BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Quinolinate synthase A (nadA) CSB-YP387780BPQ
CSB-EP387780BPQ
CSB-BP387780BPQ
CSB-MP387780BPQ
CSB-EP387780BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP387781BPQ
CSB-EP387781BPQ
CSB-BP387781BPQ
CSB-MP387781BPQ
CSB-EP387781BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Chaperone protein DnaK (dnaK), partial CSB-YP387782BPQ
CSB-EP387782BPQ
CSB-BP387782BPQ
CSB-MP387782BPQ
CSB-EP387782BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Bifunctional protein GlmU (glmU) CSB-YP387783BPQ
CSB-EP387783BPQ
CSB-BP387783BPQ
CSB-MP387783BPQ
CSB-EP387783BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 8-amino-7-oxononanoate synthase (bioF) CSB-YP387784BPQ
CSB-EP387784BPQ
CSB-BP387784BPQ
CSB-MP387784BPQ
CSB-EP387784BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Protease HtpX homolog (htpX), partial CSB-YP387785BPQ1
CSB-EP387785BPQ1
CSB-BP387785BPQ1
CSB-MP387785BPQ1
CSB-EP387785BPQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP387786BPQ
CSB-EP387786BPQ
CSB-BP387786BPQ
CSB-MP387786BPQ
CSB-EP387786BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 30S ribosomal protein S9 (rpsI) CSB-YP387787BPQ
CSB-EP387787BPQ
CSB-BP387787BPQ
CSB-MP387787BPQ
CSB-EP387787BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP387788BPQ
CSB-EP387788BPQ
CSB-BP387788BPQ
CSB-MP387788BPQ
CSB-EP387788BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP387789BPQ
CSB-EP387789BPQ
CSB-BP387789BPQ
CSB-MP387789BPQ
CSB-EP387789BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP387790BPQ1
CSB-EP387790BPQ1
CSB-BP387790BPQ1
CSB-MP387790BPQ1
CSB-EP387790BPQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Dihydrodipicolinate reductase (dapB) CSB-YP387791BPQ
CSB-EP387791BPQ
CSB-BP387791BPQ
CSB-MP387791BPQ
CSB-EP387791BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP387792BPQ
CSB-EP387792BPQ
CSB-BP387792BPQ
CSB-MP387792BPQ
CSB-EP387792BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Protein-export protein secB (secB) CSB-YP387793BPQ
CSB-EP387793BPQ
CSB-BP387793BPQ
CSB-MP387793BPQ
CSB-EP387793BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0042 nucleotide-binding protein BMA10247_2938 (BMA10247_2938) CSB-YP387794BPQ
CSB-EP387794BPQ
CSB-BP387794BPQ
CSB-MP387794BPQ
CSB-EP387794BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP387795BPQ
CSB-EP387795BPQ
CSB-BP387795BPQ
CSB-MP387795BPQ
CSB-EP387795BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP387796BPQ
CSB-EP387796BPQ
CSB-BP387796BPQ
CSB-MP387796BPQ
CSB-EP387796BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei ATP synthase subunit beta 1 (atpD1) CSB-YP387797BPQ
CSB-EP387797BPQ
CSB-BP387797BPQ
CSB-MP387797BPQ
CSB-EP387797BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Octanoyltransferase (lipB) CSB-YP387798BPQ
CSB-EP387798BPQ
CSB-BP387798BPQ
CSB-MP387798BPQ
CSB-EP387798BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>