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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia pseudomallei Adenylosuccinate synthetase (purA) CSB-YP387979BPU
CSB-EP387979BPU
CSB-BP387979BPU
CSB-MP387979BPU
CSB-EP387979BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP387980BPU
CSB-EP387980BPU
CSB-BP387980BPU
CSB-MP387980BPU
CSB-EP387980BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei UPF0133 protein BURPS668_2205 (BURPS668_2205) CSB-YP387981BPU
CSB-EP387981BPU
CSB-BP387981BPU
CSB-MP387981BPU
CSB-EP387981BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 30S ribosomal protein S6 (rpsF) CSB-YP387982BPU
CSB-EP387982BPU
CSB-BP387982BPU
CSB-MP387982BPU
CSB-EP387982BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Probable intracellular septation protein A (BURPS668_2299), partial CSB-YP387983BPU1
CSB-EP387983BPU1
CSB-BP387983BPU1
CSB-MP387983BPU1
CSB-EP387983BPU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Trigger factor (tig) CSB-YP387984BPU
CSB-EP387984BPU
CSB-BP387984BPU
CSB-MP387984BPU
CSB-EP387984BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Large-conductance mechanosensitive channel (mscL), partial CSB-YP387985BPU1
CSB-EP387985BPU1
CSB-BP387985BPU1
CSB-MP387985BPU1
CSB-EP387985BPU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Putative phosphotransferase BURPS668_2420 (BURPS668_2420) CSB-YP387986BPU
CSB-EP387986BPU
CSB-BP387986BPU
CSB-MP387986BPU
CSB-EP387986BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP387987BPU
CSB-BP387987BPU
CSB-MP387987BPU
CSB-EP387987BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 30S ribosomal protein S2 (rpsB) CSB-YP387988BPU
CSB-EP387988BPU
CSB-BP387988BPU
CSB-MP387988BPU
CSB-EP387988BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP387989BPU
CSB-EP387989BPU
CSB-BP387989BPU
CSB-MP387989BPU
CSB-EP387989BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP387990BPU
CSB-EP387990BPU
CSB-BP387990BPU
CSB-MP387990BPU
CSB-EP387990BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Chaperone protein hscA homolog (hscA), partial CSB-YP387991BPU
CSB-EP387991BPU
CSB-BP387991BPU
CSB-MP387991BPU
CSB-EP387991BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Bifunctional protein FolD (folD) CSB-YP387992BPU
CSB-EP387992BPU
CSB-BP387992BPU
CSB-MP387992BPU
CSB-EP387992BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei UvrABC system protein C (uvrC), partial CSB-YP387993BPU
CSB-EP387993BPU
CSB-BP387993BPU
CSB-MP387993BPU
CSB-EP387993BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Elongation factor 4 (lepA), partial CSB-YP387994BPU
CSB-EP387994BPU
CSB-BP387994BPU
CSB-MP387994BPU
CSB-EP387994BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Phosphate acyltransferase (plsX) CSB-YP387995BPU
CSB-EP387995BPU
CSB-BP387995BPU
CSB-MP387995BPU
CSB-EP387995BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L19 (rplS) CSB-YP387996BPU
CSB-EP387996BPU
CSB-BP387996BPU
CSB-MP387996BPU
CSB-EP387996BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Ribosome maturation factor RimM (rimM) CSB-YP387997BPU
CSB-EP387997BPU
CSB-BP387997BPU
CSB-MP387997BPU
CSB-EP387997BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP387998BPU
CSB-EP387998BPU
CSB-BP387998BPU
CSB-MP387998BPU
CSB-EP387998BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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