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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptococcus sanguinis Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP387378SUR
CSB-EP387378SUR
CSB-BP387378SUR
CSB-MP387378SUR
CSB-EP387378SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri UPF0391 membrane protein Memar_0049 (Memar_0049), partial CSB-YP387379MNU
CSB-EP387379MNU
CSB-BP387379MNU
CSB-MP387379MNU
CSB-EP387379MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Acetylglutamate kinase (argB) CSB-YP387380MNU
CSB-EP387380MNU
CSB-BP387380MNU
CSB-MP387380MNU
CSB-EP387380MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Thiamine-phosphate synthase (thiE) CSB-YP387381MNU
CSB-EP387381MNU
CSB-BP387381MNU
CSB-MP387381MNU
CSB-EP387381MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri V-type ATP synthase alpha chain (atpA), partial CSB-YP387382MNU
CSB-EP387382MNU
CSB-BP387382MNU
CSB-MP387382MNU
CSB-EP387382MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri D-tyrosyl-tRNA (Tyr) deacylase CSB-YP387383MNU
CSB-EP387383MNU
CSB-BP387383MNU
CSB-MP387383MNU
CSB-EP387383MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM) CSB-YP387384MNU
CSB-EP387384MNU
CSB-BP387384MNU
CSB-MP387384MNU
CSB-EP387384MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri 30S ribosomal protein S6e (rps6e) CSB-YP387385MNU
CSB-EP387385MNU
CSB-BP387385MNU
CSB-MP387385MNU
CSB-EP387385MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Digeranylgeranylglycerophospholipid reductase (Memar_0506) CSB-YP387386MNU
CSB-EP387386MNU
CSB-BP387386MNU
CSB-MP387386MNU
CSB-EP387386MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri F420-dependent methylenetetrahydromethanopterin dehydrogenase (mtd) CSB-YP387387MNU
CSB-EP387387MNU
CSB-BP387387MNU
CSB-MP387387MNU
CSB-EP387387MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri 30S ribosomal protein S19P (rps19p) CSB-YP387388MNU
CSB-EP387388MNU
CSB-BP387388MNU
CSB-MP387388MNU
CSB-EP387388MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri 30S ribosomal protein S17P (rps17p) CSB-YP387389MNU
CSB-EP387389MNU
CSB-BP387389MNU
CSB-MP387389MNU
CSB-EP387389MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri 50S ribosomal protein L5P (rpl5p) CSB-YP387390MNU
CSB-EP387390MNU
CSB-BP387390MNU
CSB-MP387390MNU
CSB-EP387390MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Adenylate kinase (adkA) CSB-YP387391MNU
CSB-EP387391MNU
CSB-BP387391MNU
CSB-MP387391MNU
CSB-EP387391MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Probable endonuclease 4 (nfo) CSB-YP387392MNU
CSB-EP387392MNU
CSB-BP387392MNU
CSB-MP387392MNU
CSB-EP387392MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Methenyltetrahydromethanopterin cyclohydrolase (mch) CSB-YP387393MNU
CSB-EP387393MNU
CSB-BP387393MNU
CSB-MP387393MNU
CSB-EP387393MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Probable deoxycytidine triphosphate deaminase (dcd) CSB-YP387394MNU
CSB-EP387394MNU
CSB-BP387394MNU
CSB-MP387394MNU
CSB-EP387394MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Geranylgeranylglyceryl phosphate synthase (Memar_0769) CSB-YP387395MNU
CSB-EP387395MNU
CSB-BP387395MNU
CSB-MP387395MNU
CSB-EP387395MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Putative 3-methyladenine DNA glycosylase (Memar_0903) CSB-YP387396MNU
CSB-EP387396MNU
CSB-BP387396MNU
CSB-MP387396MNU
CSB-EP387396MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Translation initiation factor 5A (eIF5A) CSB-YP387397MNU
CSB-EP387397MNU
CSB-BP387397MNU
CSB-MP387397MNU
CSB-EP387397MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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