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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella baltica Protein ApaG (apaG) CSB-YP386279STL
CSB-EP386279STL
CSB-BP386279STL
CSB-MP386279STL
CSB-EP386279STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0246 protein Sbal_1048 (Sbal_1048) CSB-YP386280STL
CSB-EP386280STL
CSB-BP386280STL
CSB-MP386280STL
CSB-EP386280STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Lipoprotein signal peptidase (lspA), partial CSB-YP386281STL1
CSB-EP386281STL1
CSB-BP386281STL1
CSB-MP386281STL1
CSB-EP386281STL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP386282STL
CSB-EP386282STL
CSB-BP386282STL
CSB-MP386282STL
CSB-EP386282STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Multifunctional CCA protein (cca) CSB-YP386283STL
CSB-EP386283STL
CSB-BP386283STL
CSB-MP386283STL
CSB-EP386283STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica RNA pyrophosphohydrolase (rppH) CSB-YP386284STL
CSB-EP386284STL
CSB-BP386284STL
CSB-MP386284STL
CSB-EP386284STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribosome maturation factor RimM (rimM) CSB-YP386285STL
CSB-EP386285STL
CSB-BP386285STL
CSB-MP386285STL
CSB-EP386285STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica SsrA-binding protein (smpB) CSB-YP386286STL
CSB-EP386286STL
CSB-BP386286STL
CSB-MP386286STL
CSB-EP386286STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP386287STL
CSB-EP386287STL
CSB-BP386287STL
CSB-MP386287STL
CSB-EP386287STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Recombination-associated protein rdgC (rdgC) CSB-YP386288STL
CSB-EP386288STL
CSB-BP386288STL
CSB-MP386288STL
CSB-EP386288STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP386289STL
CSB-EP386289STL
CSB-BP386289STL
CSB-MP386289STL
CSB-EP386289STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP386290STL
CSB-EP386290STL
CSB-BP386290STL
CSB-MP386290STL
CSB-EP386290STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Homoserine O-succinyltransferase (metA) CSB-YP386291STL
CSB-EP386291STL
CSB-BP386291STL
CSB-MP386291STL
CSB-EP386291STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Trigger factor (tig) CSB-YP386292STL
CSB-EP386292STL
CSB-BP386292STL
CSB-MP386292STL
CSB-EP386292STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP386293STL
CSB-EP386293STL
CSB-BP386293STL
CSB-MP386293STL
CSB-EP386293STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP386294STL
CSB-EP386294STL
CSB-BP386294STL
CSB-MP386294STL
CSB-EP386294STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Uracil phosphoribosyltransferase (upp) CSB-YP386295STL
CSB-EP386295STL
CSB-BP386295STL
CSB-MP386295STL
CSB-EP386295STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Biotin synthase (bioB) CSB-YP386296STL
CSB-EP386296STL
CSB-BP386296STL
CSB-MP386296STL
CSB-EP386296STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP386297STL
CSB-EP386297STL
CSB-BP386297STL
CSB-MP386297STL
CSB-EP386297STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Cell division topological specificity factor (minE) CSB-YP386298STL
CSB-EP386298STL
CSB-BP386298STL
CSB-MP386298STL
CSB-EP386298STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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