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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Actinobacillus pleuropneumoniae serotype 5b S-adenosylmethionine synthase (metK) CSB-YP387899AUF
CSB-EP387899AUF
CSB-BP387899AUF
CSB-MP387899AUF
CSB-EP387899AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b DNA mismatch repair protein mutH (mutH) CSB-YP387900AUF
CSB-EP387900AUF
CSB-BP387900AUF
CSB-MP387900AUF
CSB-EP387900AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP387901AUF
CSB-EP387901AUF
CSB-BP387901AUF
CSB-MP387901AUF
CSB-EP387901AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b tRNA threonylcarbamoyladenosine biosynthesis protein RimN (rimN) CSB-YP387902AUF
CSB-EP387902AUF
CSB-BP387902AUF
CSB-MP387902AUF
CSB-EP387902AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Aspartate--tRNA ligase (aspS), partial CSB-YP387903AUF
CSB-EP387903AUF
CSB-BP387903AUF
CSB-MP387903AUF
CSB-EP387903AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP387904AUF
CSB-EP387904AUF
CSB-BP387904AUF
CSB-MP387904AUF
CSB-EP387904AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Primosomal replication protein n (priB) CSB-YP387905AUF
CSB-EP387905AUF
CSB-BP387905AUF
CSB-MP387905AUF
CSB-EP387905AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Phosphoglycerate kinase (pgk) CSB-YP387906AUF
CSB-EP387906AUF
CSB-BP387906AUF
CSB-MP387906AUF
CSB-EP387906AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP387907AUF
CSB-EP387907AUF
CSB-BP387907AUF
CSB-MP387907AUF
CSB-EP387907AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Cell division protein ZipA homolog (zipA), partial CSB-YP387908AUF
CSB-EP387908AUF
CSB-BP387908AUF
CSB-MP387908AUF
CSB-EP387908AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b UPF0263 protein APL_1348 (APL_1348) CSB-YP387909AUF
CSB-EP387909AUF
CSB-BP387909AUF
CSB-MP387909AUF
CSB-EP387909AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Membrane-bound lytic murein transglycosylase F (mltF) CSB-YP387910AUF
CSB-EP387910AUF
CSB-BP387910AUF
CSB-MP387910AUF
CSB-EP387910AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b UPF0235 protein APL_1380 (APL_1380) CSB-YP387911AUF
CSB-EP387911AUF
CSB-BP387911AUF
CSB-MP387911AUF
CSB-EP387911AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP387912AUF
CSB-EP387912AUF
CSB-BP387912AUF
CSB-MP387912AUF
CSB-EP387912AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Periplasmic nitrate reductase (napA), partial CSB-YP387913AUF
CSB-EP387913AUF
CSB-BP387913AUF
CSB-MP387913AUF
CSB-EP387913AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 30S ribosomal protein S21 (rpsU) CSB-YP387914AUF
CSB-EP387914AUF
CSB-BP387914AUF
CSB-MP387914AUF
CSB-EP387914AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Pyridoxamine kinase (pdxY) CSB-YP387915AUF
CSB-EP387915AUF
CSB-BP387915AUF
CSB-MP387915AUF
CSB-EP387915AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b UPF0210 protein APL_1491 (APL_1491) CSB-YP387916AUF
CSB-EP387916AUF
CSB-BP387916AUF
CSB-MP387916AUF
CSB-EP387916AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Valine--tRNA ligase (valS), partial CSB-YP387917AUF
CSB-EP387917AUF
CSB-BP387917AUF
CSB-MP387917AUF
CSB-EP387917AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP387918AUF
CSB-EP387918AUF
CSB-BP387918AUF
CSB-MP387918AUF
CSB-EP387918AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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