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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia pseudomallei UPF0042 nucleotide-binding protein BURPS668_0577 (BURPS668_0577) CSB-YP387959BPU
CSB-EP387959BPU
CSB-BP387959BPU
CSB-MP387959BPU
CSB-EP387959BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP387960BPU1
CSB-EP387960BPU1
CSB-BP387960BPU1
CSB-MP387960BPU1
CSB-EP387960BPU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei UPF0234 protein BURPS668_0915 (BURPS668_0915) CSB-YP387961BPU
CSB-EP387961BPU
CSB-BP387961BPU
CSB-MP387961BPU
CSB-EP387961BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei UPF0434 protein BURPS668_0926 (BURPS668_0926) CSB-YP387962BPU
CSB-EP387962BPU
CSB-BP387962BPU
CSB-MP387962BPU
CSB-EP387962BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Quinolinate synthase A (nadA) CSB-YP387963BPU
CSB-EP387963BPU
CSB-BP387963BPU
CSB-MP387963BPU
CSB-EP387963BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei UPF0758 protein BURPS668_0979 (BURPS668_0979) CSB-YP387964BPU
CSB-EP387964BPU
CSB-BP387964BPU
CSB-MP387964BPU
CSB-EP387964BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP387965BPU1
CSB-EP387965BPU1
CSB-BP387965BPU1
CSB-MP387965BPU1
CSB-EP387965BPU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Deoxycytidine triphosphate deaminase (dcd) CSB-YP387966BPU
CSB-EP387966BPU
CSB-BP387966BPU
CSB-MP387966BPU
CSB-EP387966BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP387967BPU
CSB-EP387967BPU
CSB-BP387967BPU
CSB-MP387967BPU
CSB-EP387967BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Methylglyoxal synthase (mgsA) CSB-YP387968BPU
CSB-EP387968BPU
CSB-BP387968BPU
CSB-MP387968BPU
CSB-EP387968BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 30S ribosomal protein S15 (rpsO) CSB-YP387969BPU
CSB-EP387969BPU
CSB-BP387969BPU
CSB-MP387969BPU
CSB-EP387969BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP387970BPU
CSB-EP387970BPU
CSB-BP387970BPU
CSB-MP387970BPU
CSB-EP387970BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP387971BPU
CSB-EP387971BPU
CSB-BP387971BPU
CSB-MP387971BPU
CSB-EP387971BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Phosphomethylpyrimidine synthase (thiC), partial CSB-YP387972BPU
CSB-EP387972BPU
CSB-BP387972BPU
CSB-MP387972BPU
CSB-EP387972BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Phosphoglucosamine mutase (glmM) CSB-YP387973BPU
CSB-EP387973BPU
CSB-BP387973BPU
CSB-MP387973BPU
CSB-EP387973BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei UPF0145 protein BURPS668_1644 (BURPS668_1644) CSB-YP387974BPU
CSB-EP387974BPU
CSB-BP387974BPU
CSB-MP387974BPU
CSB-EP387974BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Ribosome maturation factor RimP (rimP) CSB-YP387975BPU
CSB-EP387975BPU
CSB-BP387975BPU
CSB-MP387975BPU
CSB-EP387975BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei tRNA pseudouridine synthase B (truB) CSB-YP387976BPU
CSB-EP387976BPU
CSB-BP387976BPU
CSB-MP387976BPU
CSB-EP387976BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Putative arginyl-tRNA--protein transferase (ate) CSB-YP387977BPU
CSB-EP387977BPU
CSB-BP387977BPU
CSB-MP387977BPU
CSB-EP387977BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP387978BPU
CSB-EP387978BPU
CSB-BP387978BPU
CSB-MP387978BPU
CSB-EP387978BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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