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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium sp. Bifunctional protein FolD (folD) CSB-YP388561MOL
CSB-EP388561MOL
CSB-BP388561MOL
CSB-MP388561MOL
CSB-EP388561MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Protein translocase subunit SecA 1 (secA1), partial CSB-YP388562MOL
CSB-EP388562MOL
CSB-BP388562MOL
CSB-MP388562MOL
CSB-EP388562MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP388563MOL
CSB-EP388563MOL
CSB-BP388563MOL
CSB-MP388563MOL
CSB-EP388563MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B, partial CSB-YP388564MOL
CSB-EP388564MOL
CSB-BP388564MOL
CSB-MP388564MOL
CSB-EP388564MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP388565MOL
CSB-EP388565MOL
CSB-BP388565MOL
CSB-MP388565MOL
CSB-EP388565MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP388566MOL
CSB-EP388566MOL
CSB-BP388566MOL
CSB-MP388566MOL
CSB-EP388566MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Uridylate kinase (pyrH) CSB-YP388567MOL
CSB-EP388567MOL
CSB-BP388567MOL
CSB-MP388567MOL
CSB-EP388567MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Probable malate:quinone oxidoreductase (mqo), partial CSB-YP388568MOL
CSB-EP388568MOL
CSB-BP388568MOL
CSB-MP388568MOL
CSB-EP388568MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Ribosome-binding factor A (rbfA) CSB-YP388569MOL
CSB-EP388569MOL
CSB-BP388569MOL
CSB-MP388569MOL
CSB-EP388569MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP388570MOL
CSB-EP388570MOL
CSB-BP388570MOL
CSB-MP388570MOL
CSB-EP388570MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Thymidylate synthase thyX (thyX) CSB-YP388571MOL
CSB-EP388571MOL
CSB-BP388571MOL
CSB-MP388571MOL
CSB-EP388571MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB, partial CSB-YP388572MOL
CSB-EP388572MOL
CSB-BP388572MOL
CSB-MP388572MOL
CSB-EP388572MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Na (+)/H (+) antiporter nhaA 2, partial CSB-YP388573MOL
CSB-EP388573MOL
CSB-BP388573MOL
CSB-MP388573MOL
CSB-EP388573MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Threonine--tRNA ligase (thrS), partial CSB-YP388574MOL
CSB-EP388574MOL
CSB-BP388574MOL
CSB-MP388574MOL
CSB-EP388574MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP388575MOL
CSB-EP388575MOL
CSB-BP388575MOL
CSB-MP388575MOL
CSB-EP388575MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Gas vesicle structural protein (gvpA) CSB-YP388576MOL
CSB-EP388576MOL
CSB-BP388576MOL
CSB-MP388576MOL
CSB-EP388576MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Aspartate carbamoyltransferase (pyrB) CSB-YP388577MOL
CSB-EP388577MOL
CSB-BP388577MOL
CSB-MP388577MOL
CSB-EP388577MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Pup--protein ligase (pafA) CSB-YP388578MOL
CSB-EP388578MOL
CSB-BP388578MOL
CSB-MP388578MOL
CSB-EP388578MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Urease subunit beta (ureB) CSB-YP388579MOL
CSB-EP388579MOL
CSB-BP388579MOL
CSB-MP388579MOL
CSB-EP388579MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Urease subunit gamma (ureA) CSB-YP388580MOL
CSB-EP388580MOL
CSB-BP388580MOL
CSB-MP388580MOL
CSB-EP388580MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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