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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella loihica Iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP388761STO
CSB-EP388761STO
CSB-BP388761STO
CSB-MP388761STO
CSB-EP388761STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP388762STO
CSB-EP388762STO
CSB-BP388762STO
CSB-MP388762STO
CSB-EP388762STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica tRNA (guanine-N (1)-)-methyltransferase CSB-YP388763STO
CSB-EP388763STO
CSB-BP388763STO
CSB-MP388763STO
CSB-EP388763STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Probable Fe (2+)-trafficking protein CSB-YP388764STO
CSB-EP388764STO
CSB-BP388764STO
CSB-MP388764STO
CSB-EP388764STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica N utilization substance protein B homolog (nusB) CSB-YP388765STO
CSB-EP388765STO
CSB-BP388765STO
CSB-MP388765STO
CSB-EP388765STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP388766STO
CSB-EP388766STO
CSB-BP388766STO
CSB-MP388766STO
CSB-EP388766STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP388767STO
CSB-EP388767STO
CSB-BP388767STO
CSB-MP388767STO
CSB-EP388767STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Membrane-bound lytic murein transglycosylase F (mltF) CSB-YP388768STO
CSB-EP388768STO
CSB-BP388768STO
CSB-MP388768STO
CSB-EP388768STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Protein phosphatase CheZ (cheZ) CSB-YP388769STO
CSB-EP388769STO
CSB-BP388769STO
CSB-MP388769STO
CSB-EP388769STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Uracil phosphoribosyltransferase (upp) CSB-YP388770STO
CSB-EP388770STO
CSB-BP388770STO
CSB-MP388770STO
CSB-EP388770STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Translation initiation factor IF-1 (infA) CSB-YP388771STO
CSB-EP388771STO
CSB-BP388771STO
CSB-MP388771STO
CSB-EP388771STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica UPF0502 protein Shew_1617 (Shew_1617) CSB-YP388772STO
CSB-EP388772STO
CSB-BP388772STO
CSB-MP388772STO
CSB-EP388772STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP388773STO
CSB-EP388773STO
CSB-BP388773STO
CSB-MP388773STO
CSB-EP388773STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Protease HtpX (htpX), partial CSB-YP388774STO1
CSB-EP388774STO1
CSB-BP388774STO1
CSB-MP388774STO1
CSB-EP388774STO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP388775STO
CSB-EP388775STO
CSB-BP388775STO
CSB-MP388775STO
CSB-EP388775STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Elongation factor P (efp) CSB-YP388776STO
CSB-EP388776STO
CSB-BP388776STO
CSB-MP388776STO
CSB-EP388776STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP388777STO
CSB-EP388777STO
CSB-BP388777STO
CSB-MP388777STO
CSB-EP388777STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP388778STO
CSB-EP388778STO
CSB-BP388778STO
CSB-MP388778STO
CSB-EP388778STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 50S ribosomal protein L35 (rpmI) CSB-YP388779STO
CSB-EP388779STO
CSB-BP388779STO
CSB-MP388779STO
CSB-EP388779STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Electron transport complex protein RnfG (rnfG), partial CSB-YP388780STO
CSB-EP388780STO
CSB-BP388780STO
CSB-MP388780STO
CSB-EP388780STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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