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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium sp. 50S ribosomal protein L30 (rpmD) CSB-YP388460MOL
CSB-EP388460MOL
CSB-BP388460MOL
CSB-MP388460MOL
CSB-EP388460MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 30S ribosomal protein S13 (rpsM) CSB-YP388461MOL
CSB-EP388461MOL
CSB-BP388461MOL
CSB-MP388461MOL
CSB-EP388461MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 50S ribosomal protein L17 (rplQ) CSB-YP388462MOL
CSB-EP388462MOL
CSB-BP388462MOL
CSB-MP388462MOL
CSB-EP388462MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 60 kDa chaperonin 2 (groL2), partial CSB-YP388463MOL
CSB-EP388463MOL
CSB-BP388463MOL
CSB-MP388463MOL
CSB-EP388463MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 2-phospho-L-lactate transferase (cofD) CSB-YP388464MOL
CSB-EP388464MOL
CSB-BP388464MOL
CSB-MP388464MOL
CSB-EP388464MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP388465MOL
CSB-EP388465MOL
CSB-BP388465MOL
CSB-MP388465MOL
CSB-EP388465MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP388466MOL1
CSB-EP388466MOL1
CSB-BP388466MOL1
CSB-MP388466MOL1
CSB-EP388466MOL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP388467MOL
CSB-EP388467MOL
CSB-BP388467MOL
CSB-MP388467MOL
CSB-EP388467MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Protein nrdI (nrdI) CSB-YP388468MOL
CSB-EP388468MOL
CSB-BP388468MOL
CSB-MP388468MOL
CSB-EP388468MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. DNA ligase (ligA), partial CSB-YP388469MOL
CSB-EP388469MOL
CSB-BP388469MOL
CSB-MP388469MOL
CSB-EP388469MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Glutamate--tRNA ligase (gltX) CSB-YP388470MOL
CSB-EP388470MOL
CSB-BP388470MOL
CSB-MP388470MOL
CSB-EP388470MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP388471MOL
CSB-EP388471MOL
CSB-BP388471MOL
CSB-MP388471MOL
CSB-EP388471MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Uncharacterized oxidoreductase Mjls_1918 (Mjls_1918) CSB-YP388472MOL
CSB-EP388472MOL
CSB-BP388472MOL
CSB-MP388472MOL
CSB-EP388472MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Acylphosphatase (acyP) CSB-YP388473MOL
CSB-EP388473MOL
CSB-BP388473MOL
CSB-MP388473MOL
CSB-EP388473MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Ribosome maturation factor RimM (rimM) CSB-YP388474MOL
CSB-EP388474MOL
CSB-BP388474MOL
CSB-MP388474MOL
CSB-EP388474MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. UPF0102 protein Mjls_1965 (Mjls_1965) CSB-YP388475MOL
CSB-EP388475MOL
CSB-BP388475MOL
CSB-MP388475MOL
CSB-EP388475MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 30S ribosomal protein S2 (rpsB) CSB-YP388476MOL
CSB-EP388476MOL
CSB-BP388476MOL
CSB-MP388476MOL
CSB-EP388476MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Cobyric acid synthase (cobQ) CSB-YP388477MOL
CSB-EP388477MOL
CSB-BP388477MOL
CSB-MP388477MOL
CSB-EP388477MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Ribosome maturation factor RimP (rimP) CSB-YP388478MOL
CSB-EP388478MOL
CSB-BP388478MOL
CSB-MP388478MOL
CSB-EP388478MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP388479MOL
CSB-EP388479MOL
CSB-BP388479MOL
CSB-MP388479MOL
CSB-EP388479MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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