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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella loihica Tryptophan synthase beta chain (trpB) CSB-YP388661STO
CSB-EP388661STO
CSB-BP388661STO
CSB-MP388661STO
CSB-EP388661STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Co-chaperone protein hscB homolog (hscB) CSB-YP388662STO
CSB-EP388662STO
CSB-BP388662STO
CSB-MP388662STO
CSB-EP388662STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Acetate kinase (ackA) CSB-YP388663STO
CSB-EP388663STO
CSB-BP388663STO
CSB-MP388663STO
CSB-EP388663STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP388664STO
CSB-EP388664STO
CSB-BP388664STO
CSB-MP388664STO
CSB-EP388664STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Bifunctional protein FolD (folD) CSB-YP388665STO
CSB-EP388665STO
CSB-BP388665STO
CSB-MP388665STO
CSB-EP388665STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Proline--tRNA ligase (proS), partial CSB-YP388666STO
CSB-EP388666STO
CSB-BP388666STO
CSB-MP388666STO
CSB-EP388666STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Lipid-A-disaccharide synthase (lpxB) CSB-YP388667STO
CSB-EP388667STO
CSB-BP388667STO
CSB-MP388667STO
CSB-EP388667STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Uridylate kinase (pyrH) CSB-YP388668STO
CSB-EP388668STO
CSB-BP388668STO
CSB-MP388668STO
CSB-EP388668STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP388669STO
CSB-EP388669STO
CSB-BP388669STO
CSB-MP388669STO
CSB-EP388669STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Recombination-associated protein rdgC (rdgC) CSB-YP388670STO
CSB-EP388670STO
CSB-BP388670STO
CSB-MP388670STO
CSB-EP388670STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica tRNA sulfurtransferase (thiI) CSB-YP388671STO
CSB-EP388671STO
CSB-BP388671STO
CSB-MP388671STO
CSB-EP388671STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Protein grpE (grpE) CSB-YP388672STO
CSB-EP388672STO
CSB-BP388672STO
CSB-MP388672STO
CSB-EP388672STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP388673STO
CSB-EP388673STO
CSB-BP388673STO
CSB-MP388673STO
CSB-EP388673STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP388674STO
CSB-EP388674STO
CSB-BP388674STO
CSB-MP388674STO
CSB-EP388674STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Phosphoglucosamine mutase (glmM) CSB-YP388675STO
CSB-EP388675STO
CSB-BP388675STO
CSB-MP388675STO
CSB-EP388675STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Chaperone protein DnaK (dnaK), partial CSB-YP388676STO
CSB-EP388676STO
CSB-BP388676STO
CSB-MP388676STO
CSB-EP388676STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica S-ribosylhomocysteine lyase (luxS) CSB-YP388677STO
CSB-EP388677STO
CSB-BP388677STO
CSB-MP388677STO
CSB-EP388677STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP388678STO
CSB-EP388678STO
CSB-BP388678STO
CSB-MP388678STO
CSB-EP388678STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Glutamyl-tRNA reductase (hemA) CSB-YP388679STO
CSB-EP388679STO
CSB-BP388679STO
CSB-MP388679STO
CSB-EP388679STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP388680STO
CSB-EP388680STO
CSB-BP388680STO
CSB-MP388680STO
CSB-EP388680STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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