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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium sp. Adenylosuccinate synthetase (purA) CSB-YP388260MOL
CSB-EP388260MOL
CSB-BP388260MOL
CSB-MP388260MOL
CSB-EP388260MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Carboxylate-amine ligase Mjls_0563 (Mjls_0563) CSB-YP388261MOL
CSB-EP388261MOL
CSB-BP388261MOL
CSB-MP388261MOL
CSB-EP388261MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 60 kDa chaperonin 1 (groL1), partial CSB-YP388262MOL
CSB-EP388262MOL
CSB-BP388262MOL
CSB-MP388262MOL
CSB-EP388262MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP388263MOL
CSB-EP388263MOL
CSB-BP388263MOL
CSB-MP388263MOL
CSB-EP388263MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Protease HtpX homolog (htpX), partial CSB-YP388264MOL1
CSB-EP388264MOL1
CSB-BP388264MOL1
CSB-MP388264MOL1
CSB-EP388264MOL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 50S ribosomal protein L1 (rplA) CSB-YP388265MOL
CSB-EP388265MOL
CSB-BP388265MOL
CSB-MP388265MOL
CSB-EP388265MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 30S ribosomal protein S12 (rpsL) CSB-YP388266MOL
CSB-EP388266MOL
CSB-BP388266MOL
CSB-MP388266MOL
CSB-EP388266MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 30S ribosomal protein S10 (rpsJ) CSB-YP388267MOL
CSB-EP388267MOL
CSB-BP388267MOL
CSB-MP388267MOL
CSB-EP388267MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 30S ribosomal protein S19 (rpsS) CSB-YP388268MOL
CSB-EP388268MOL
CSB-BP388268MOL
CSB-MP388268MOL
CSB-EP388268MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 30S ribosomal protein S3 (rpsC) CSB-YP388269MOL
CSB-EP388269MOL
CSB-BP388269MOL
CSB-MP388269MOL
CSB-EP388269MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 50S ribosomal protein L14 (rplN) CSB-YP388270MOL
CSB-EP388270MOL
CSB-BP388270MOL
CSB-MP388270MOL
CSB-EP388270MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 30S ribosomal protein S14 type Z (rpsZ) CSB-YP388271MOL
CSB-EP388271MOL
CSB-BP388271MOL
CSB-MP388271MOL
CSB-EP388271MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Putative S-adenosyl-L-methionine-dependent methyltransferase Mjls_1072 (Mjls_1072) CSB-YP388272MOL
CSB-EP388272MOL
CSB-BP388272MOL
CSB-MP388272MOL
CSB-EP388272MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Translation initiation factor IF-1 (infA) CSB-YP388273MOL
CSB-EP388273MOL
CSB-BP388273MOL
CSB-MP388273MOL
CSB-EP388273MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 30S ribosomal protein S11 (rpsK) CSB-YP388274MOL
CSB-EP388274MOL
CSB-BP388274MOL
CSB-MP388274MOL
CSB-EP388274MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 30S ribosomal protein S4 (rpsD) CSB-YP388275MOL
CSB-EP388275MOL
CSB-BP388275MOL
CSB-MP388275MOL
CSB-EP388275MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Phosphoglucosamine mutase (glmM) CSB-YP388276MOL
CSB-EP388276MOL
CSB-BP388276MOL
CSB-MP388276MOL
CSB-EP388276MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Maf-like protein Mjls_1328 (Mjls_1328) CSB-YP388277MOL
CSB-EP388277MOL
CSB-BP388277MOL
CSB-MP388277MOL
CSB-EP388277MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP388278MOL
CSB-EP388278MOL
CSB-BP388278MOL
CSB-MP388278MOL
CSB-EP388278MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP388279MOL
CSB-EP388279MOL
CSB-BP388279MOL
CSB-MP388279MOL
CSB-EP388279MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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