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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Macaca mulatta Low affinity immunoglobulin gamma Fc region receptor III (FCGR3), partial CSB-YP388701MOW
CSB-EP388701MOW
CSB-BP388701MOW
CSB-MP388701MOW
CSB-EP388701MOW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabies virus Nucleoprotein (N) CSB-YP388702RIE
CSB-EP388702RIE
CSB-BP388702RIE
CSB-MP388702RIE
CSB-EP388702RIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabies virus Matrix protein (M) CSB-YP388703RIE
CSB-EP388703RIE
CSB-BP388703RIE
CSB-MP388703RIE
CSB-EP388703RIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Putative ecotin-like protein (WH7805_09909) CSB-YP388704FQB
CSB-EP388704FQB
CSB-BP388704FQB
CSB-MP388704FQB
CSB-EP388704FQB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Leucine-rich repeat and death domain-containing protein 1 (LRRD1), partial CSB-YP388705HU
CSB-EP388705HU
CSB-BP388705HU
CSB-MP388705HU
CSB-EP388705HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative postmeiotic segregation increased 2-like protein 1 (PMS2P1) CSB-YP388706HU
CSB-EP388706HU
CSB-BP388706HU
CSB-MP388706HU
CSB-EP388706HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. UPF0353 protein Mjls_2492 (Mjls_2492), partial CSB-YP388707MOL1
CSB-EP388707MOL1
CSB-BP388707MOL1
CSB-MP388707MOL1
CSB-EP388707MOL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Sec-independent protein translocase protein TatA (tatA), partial CSB-YP388708MOL
CSB-EP388708MOL
CSB-BP388708MOL
CSB-MP388708MOL
CSB-EP388708MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Catalase-peroxidase (katG), partial CSB-YP388709MOL
CSB-EP388709MOL
CSB-BP388709MOL
CSB-MP388709MOL
CSB-EP388709MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Phthiocerol/phthiodiolone dimycocerosyl transferase (papA5) CSB-YP388710MOL
CSB-EP388710MOL
CSB-BP388710MOL
CSB-MP388710MOL
CSB-EP388710MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP388711MOL
CSB-EP388711MOL
CSB-BP388711MOL
CSB-MP388711MOL
CSB-EP388711MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Quinolinate synthase A (nadA) CSB-YP388712MOL
CSB-EP388712MOL
CSB-BP388712MOL
CSB-MP388712MOL
CSB-EP388712MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP388713MOL
CSB-EP388713MOL
CSB-BP388713MOL
CSB-MP388713MOL
CSB-EP388713MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. ATP phosphoribosyltransferase (hisG) CSB-YP388714MOL
CSB-EP388714MOL
CSB-BP388714MOL
CSB-MP388714MOL
CSB-EP388714MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP388715MOL
CSB-EP388715MOL
CSB-BP388715MOL
CSB-MP388715MOL
CSB-EP388715MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Protein MraZ (mraZ) CSB-YP388716MOL
CSB-EP388716MOL
CSB-BP388716MOL
CSB-MP388716MOL
CSB-EP388716MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Probable cytosol aminopeptidase (pepA), partial CSB-YP388717MOL
CSB-EP388717MOL
CSB-BP388717MOL
CSB-MP388717MOL
CSB-EP388717MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP388718MOL
CSB-EP388718MOL
CSB-BP388718MOL
CSB-MP388718MOL
CSB-EP388718MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 30S ribosomal protein S20 (rpsT) CSB-YP388719MOL
CSB-EP388719MOL
CSB-BP388719MOL
CSB-MP388719MOL
CSB-EP388719MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. GTPase obg (obg) CSB-YP388720MOL
CSB-EP388720MOL
CSB-BP388720MOL
CSB-MP388720MOL
CSB-EP388720MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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