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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium sp. Leucine--tRNA ligase (leuS), partial CSB-YP388901MOL
CSB-EP388901MOL
CSB-BP388901MOL
CSB-MP388901MOL
CSB-EP388901MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Ribonuclease P protein component (rnpA) CSB-YP388902MOL
CSB-EP388902MOL
CSB-BP388902MOL
CSB-MP388902MOL
CSB-EP388902MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica DNA replication and repair protein recF (recF) CSB-YP388903STO
CSB-EP388903STO
CSB-BP388903STO
CSB-MP388903STO
CSB-EP388903STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP388904STO
CSB-EP388904STO
CSB-BP388904STO
CSB-MP388904STO
CSB-EP388904STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica tRNA 2-selenouridine synthase (selU) CSB-YP388905STO
CSB-EP388905STO
CSB-BP388905STO
CSB-MP388905STO
CSB-EP388905STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Elongation factor Tu 1 (tuf1) CSB-YP388906STO
CSB-EP388906STO
CSB-BP388906STO
CSB-MP388906STO
CSB-EP388906STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP388907STO
CSB-EP388907STO
CSB-BP388907STO
CSB-MP388907STO
CSB-EP388907STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 30S ribosomal protein S12 (rpsL) CSB-YP388908STO
CSB-EP388908STO
CSB-BP388908STO
CSB-MP388908STO
CSB-EP388908STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 30S ribosomal protein S10 (rpsJ) CSB-YP388909STO
CSB-EP388909STO
CSB-BP388909STO
CSB-MP388909STO
CSB-EP388909STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 50S ribosomal protein L23 (rplW) CSB-YP388910STO
CSB-EP388910STO
CSB-BP388910STO
CSB-MP388910STO
CSB-EP388910STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 30S ribosomal protein S17 (rpsQ) CSB-YP388911STO
CSB-EP388911STO
CSB-BP388911STO
CSB-MP388911STO
CSB-EP388911STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 50S ribosomal protein L5 (rplE) CSB-YP388912STO
CSB-EP388912STO
CSB-BP388912STO
CSB-MP388912STO
CSB-EP388912STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 50S ribosomal protein L6 (rplF) CSB-YP388913STO
CSB-EP388913STO
CSB-BP388913STO
CSB-MP388913STO
CSB-EP388913STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 50S ribosomal protein L15 (rplO) CSB-YP388914STO
CSB-EP388914STO
CSB-BP388914STO
CSB-MP388914STO
CSB-EP388914STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP388915STO
CSB-EP388915STO
CSB-BP388915STO
CSB-MP388915STO
CSB-EP388915STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica UPF0102 protein Shew_0226 (Shew_0226) CSB-YP388916STO
CSB-EP388916STO
CSB-BP388916STO
CSB-MP388916STO
CSB-EP388916STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Cell division protein ZapB (zapB) CSB-YP388917STO
CSB-EP388917STO
CSB-BP388917STO
CSB-MP388917STO
CSB-EP388917STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Dihydroxy-acid dehydratase (ilvD), partial CSB-YP388918STO
CSB-EP388918STO
CSB-BP388918STO
CSB-MP388918STO
CSB-EP388918STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica ATP-dependent RNA helicase rhlB (rhlB) CSB-YP388919STO
CSB-EP388919STO
CSB-BP388919STO
CSB-MP388919STO
CSB-EP388919STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP388920STO
CSB-EP388920STO
CSB-BP388920STO
CSB-MP388920STO
CSB-EP388920STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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