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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Rhodobacter sphaeroides NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP388500RIJ
CSB-EP388500RIJ
CSB-BP388500RIJ
CSB-MP388500RIJ
CSB-EP388500RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Type III pantothenate kinase (coaX) CSB-YP388501RIJ
CSB-EP388501RIJ
CSB-BP388501RIJ
CSB-MP388501RIJ
CSB-EP388501RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 5'-nucleotidase surE (surE) CSB-YP388502RIJ
CSB-EP388502RIJ
CSB-BP388502RIJ
CSB-MP388502RIJ
CSB-EP388502RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP388503RIJ
CSB-EP388503RIJ
CSB-BP388503RIJ
CSB-MP388503RIJ
CSB-EP388503RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Putative arginyl-tRNA--protein transferase (ate) CSB-YP388504RIJ
CSB-EP388504RIJ
CSB-BP388504RIJ
CSB-MP388504RIJ
CSB-EP388504RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP388505RIJ
CSB-EP388505RIJ
CSB-BP388505RIJ
CSB-MP388505RIJ
CSB-EP388505RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP388506RIJ
CSB-EP388506RIJ
CSB-BP388506RIJ
CSB-MP388506RIJ
CSB-EP388506RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP388507RIJ
CSB-EP388507RIJ
CSB-BP388507RIJ
CSB-MP388507RIJ
CSB-EP388507RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP388508RIJ
CSB-EP388508RIJ
CSB-BP388508RIJ
CSB-MP388508RIJ
CSB-EP388508RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Phosphopantetheine adenylyltransferase (coaD) CSB-YP388509RIJ
CSB-EP388509RIJ
CSB-BP388509RIJ
CSB-MP388509RIJ
CSB-EP388509RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Homoserine O-succinyltransferase (metA) CSB-YP388510RIJ
CSB-EP388510RIJ
CSB-BP388510RIJ
CSB-MP388510RIJ
CSB-EP388510RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Flagellar basal body rod protein FlgB (flgB) CSB-YP388511RIJ
CSB-EP388511RIJ
CSB-BP388511RIJ
CSB-MP388511RIJ
CSB-EP388511RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides NADH-quinone oxidoreductase subunit I 2 (nuoI2) CSB-YP388512RIJ
CSB-EP388512RIJ
CSB-BP388512RIJ
CSB-MP388512RIJ
CSB-EP388512RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Trigger factor (tig) CSB-YP388513RIJ
CSB-EP388513RIJ
CSB-BP388513RIJ
CSB-MP388513RIJ
CSB-EP388513RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein (bchL) CSB-YP388514RIJ
CSB-EP388514RIJ
CSB-BP388514RIJ
CSB-MP388514RIJ
CSB-EP388514RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Urease subunit alpha (ureC), partial CSB-YP388515RIJ
CSB-EP388515RIJ
CSB-BP388515RIJ
CSB-MP388515RIJ
CSB-EP388515RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Adenylosuccinate synthetase (purA) CSB-YP388516RIJ
CSB-EP388516RIJ
CSB-BP388516RIJ
CSB-MP388516RIJ
CSB-EP388516RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides UPF0145 protein Rsph17029_2093 (Rsph17029_2093) CSB-YP388517RIJ
CSB-EP388517RIJ
CSB-BP388517RIJ
CSB-MP388517RIJ
CSB-EP388517RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP388518RIJ
CSB-EP388518RIJ
CSB-BP388518RIJ
CSB-MP388518RIJ
CSB-EP388518RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP388519RIJ
CSB-EP388519RIJ
CSB-BP388519RIJ
CSB-MP388519RIJ
CSB-EP388519RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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