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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium sp. Lysylphosphatidylglycerol biosynthesis bifunctional protein LysX (lysX), partial CSB-YP388581MOL
CSB-EP388581MOL
CSB-BP388581MOL
CSB-MP388581MOL
CSB-EP388581MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP388582MOL
CSB-EP388582MOL
CSB-BP388582MOL
CSB-MP388582MOL
CSB-EP388582MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Putative 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase (Mjls_3094) CSB-YP388583MOL
CSB-EP388583MOL
CSB-BP388583MOL
CSB-MP388583MOL
CSB-EP388583MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Proteasome subunit beta (prcB) CSB-YP388584MOL
CSB-EP388584MOL
CSB-BP388584MOL
CSB-MP388584MOL
CSB-EP388584MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (mshC) CSB-YP388585MOL
CSB-EP388585MOL
CSB-BP388585MOL
CSB-MP388585MOL
CSB-EP388585MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Dihydroorotate dehydrogenase (quinone) CSB-YP388586MOL
CSB-EP388586MOL
CSB-BP388586MOL
CSB-MP388586MOL
CSB-EP388586MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Cell division protein SepF (sepF) CSB-YP388587MOL
CSB-EP388587MOL
CSB-BP388587MOL
CSB-MP388587MOL
CSB-EP388587MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP388588MOL
CSB-EP388588MOL
CSB-BP388588MOL
CSB-MP388588MOL
CSB-EP388588MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Cobalamin synthase (cobS), partial CSB-YP388589MOL
CSB-EP388589MOL
CSB-BP388589MOL
CSB-MP388589MOL
CSB-EP388589MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Probable rRNA maturation factor (Mjls_3466) CSB-YP388590MOL
CSB-EP388590MOL
CSB-BP388590MOL
CSB-MP388590MOL
CSB-EP388590MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Gamma-glutamyl phosphate reductase (proA) CSB-YP388591MOL
CSB-EP388591MOL
CSB-BP388591MOL
CSB-MP388591MOL
CSB-EP388591MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP388592MOL
CSB-EP388592MOL
CSB-BP388592MOL
CSB-MP388592MOL
CSB-EP388592MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP388593MOL
CSB-EP388593MOL
CSB-BP388593MOL
CSB-MP388593MOL
CSB-EP388593MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP388594MOL
CSB-EP388594MOL
CSB-BP388594MOL
CSB-MP388594MOL
CSB-EP388594MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 2-oxoglutarate decarboxylase (kgd), partial CSB-YP388595MOL
CSB-EP388595MOL
CSB-BP388595MOL
CSB-MP388595MOL
CSB-EP388595MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 4-hydroxy-2-oxovalerate aldolase 1 (Mjls_4224) CSB-YP388596MOL
CSB-EP388596MOL
CSB-BP388596MOL
CSB-MP388596MOL
CSB-EP388596MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Putative pterin-4-alpha-carbinolamine dehydratase (Mjls_4273) CSB-YP388597MOL
CSB-EP388597MOL
CSB-BP388597MOL
CSB-MP388597MOL
CSB-EP388597MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP388598MOL
CSB-EP388598MOL
CSB-BP388598MOL
CSB-MP388598MOL
CSB-EP388598MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Enolase (eno) CSB-YP388599MOL
CSB-EP388599MOL
CSB-BP388599MOL
CSB-MP388599MOL
CSB-EP388599MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Response regulator mprA (mprA) CSB-YP388600MOL
CSB-EP388600MOL
CSB-BP388600MOL
CSB-MP388600MOL
CSB-EP388600MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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