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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharopolyspora erythraea Pantothenate synthetase (panC) CSB-YP389182STB
CSB-EP389182STB
CSB-BP389182STB
CSB-MP389182STB
CSB-EP389182STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Type III pantothenate kinase (coaX) CSB-YP389183STB
CSB-EP389183STB
CSB-BP389183STB
CSB-MP389183STB
CSB-EP389183STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Cysteine--tRNA ligase (cysS) CSB-YP389184STB
CSB-EP389184STB
CSB-BP389184STB
CSB-MP389184STB
CSB-EP389184STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Urease subunit gamma (ureA) CSB-YP389185STB
CSB-EP389185STB
CSB-BP389185STB
CSB-MP389185STB
CSB-EP389185STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Urease accessory protein UreD 1 (ureD1) CSB-YP389186STB
CSB-EP389186STB
CSB-BP389186STB
CSB-MP389186STB
CSB-EP389186STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 50S ribosomal protein L33 1 (rpmG1) CSB-YP389187STB
CSB-EP389187STB
CSB-BP389187STB
CSB-MP389187STB
CSB-EP389187STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Methionine--tRNA ligase (metG), partial CSB-YP389188STB
CSB-EP389188STB
CSB-BP389188STB
CSB-MP389188STB
CSB-EP389188STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Enolase (eno) CSB-YP389189STB
CSB-EP389189STB
CSB-BP389189STB
CSB-MP389189STB
CSB-EP389189STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP389190STB
CSB-EP389190STB
CSB-BP389190STB
CSB-MP389190STB
CSB-EP389190STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea UPF0182 protein SACE_1102 (SACE_1102), partial CSB-YP389191STB
CSB-EP389191STB
CSB-BP389191STB
CSB-MP389191STB
CSB-EP389191STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Glutamate racemase (murI) CSB-YP389192STB
CSB-EP389192STB
CSB-BP389192STB
CSB-MP389192STB
CSB-EP389192STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Protoheme IX farnesyltransferase 1 (ctaB1), partial CSB-YP389193STB
CSB-EP389193STB
CSB-BP389193STB
CSB-MP389193STB
CSB-EP389193STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Gamma-glutamyl phosphate reductase (proA) CSB-YP389194STB
CSB-EP389194STB
CSB-BP389194STB
CSB-MP389194STB
CSB-EP389194STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Probable rRNA maturation factor (SACE_1484) CSB-YP389195STB
CSB-EP389195STB
CSB-BP389195STB
CSB-MP389195STB
CSB-EP389195STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea UPF0317 protein SACE_1553 (SACE_1553) CSB-YP389196STB
CSB-EP389196STB
CSB-BP389196STB
CSB-MP389196STB
CSB-EP389196STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Protein RecA (recA) CSB-YP389197STB
CSB-EP389197STB
CSB-BP389197STB
CSB-MP389197STB
CSB-EP389197STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Putative S-adenosyl-L-methionine-dependent methyltransferase SACE_1742 (SACE_1742) CSB-YP389198STB
CSB-EP389198STB
CSB-BP389198STB
CSB-MP389198STB
CSB-EP389198STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP389199STB
CSB-EP389199STB
CSB-BP389199STB
CSB-MP389199STB
CSB-EP389199STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Alanine--tRNA ligase (alaS), partial CSB-YP389200STB
CSB-EP389200STB
CSB-BP389200STB
CSB-MP389200STB
CSB-EP389200STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea 3-dehydroquinate dehydratase (aroQ) CSB-YP389201STB
CSB-EP389201STB
CSB-BP389201STB
CSB-MP389201STB
CSB-EP389201STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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