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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella loihica UPF0250 protein Shew_2940 (Shew_2940) CSB-YP388981STO
CSB-EP388981STO
CSB-BP388981STO
CSB-MP388981STO
CSB-EP388981STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP388982STO
CSB-EP388982STO
CSB-BP388982STO
CSB-MP388982STO
CSB-EP388982STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Alanine racemase (alr) CSB-YP388983STO
CSB-EP388983STO
CSB-BP388983STO
CSB-MP388983STO
CSB-EP388983STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 3-dehydroquinate dehydratase (aroQ) CSB-YP388984STO
CSB-EP388984STO
CSB-BP388984STO
CSB-MP388984STO
CSB-EP388984STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP388985STO
CSB-EP388985STO
CSB-BP388985STO
CSB-MP388985STO
CSB-EP388985STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Protein translocase subunit SecA (secA), partial CSB-YP388986STO
CSB-EP388986STO
CSB-BP388986STO
CSB-MP388986STO
CSB-EP388986STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Glycerol kinase (glpK) CSB-YP388987STO
CSB-EP388987STO
CSB-BP388987STO
CSB-MP388987STO
CSB-EP388987STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 50S ribosomal protein L33 (rpmG) CSB-YP388988STO
CSB-EP388988STO
CSB-BP388988STO
CSB-MP388988STO
CSB-EP388988STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 50S ribosomal protein L28 (rpmB) CSB-YP388989STO
CSB-EP388989STO
CSB-BP388989STO
CSB-MP388989STO
CSB-EP388989STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP388990STO
CSB-EP388990STO
CSB-BP388990STO
CSB-MP388990STO
CSB-EP388990STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Ribonuclease PH (rph) CSB-YP388991STO
CSB-EP388991STO
CSB-BP388991STO
CSB-MP388991STO
CSB-EP388991STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica D-tyrosyl-tRNA (Tyr) deacylase CSB-YP388992STO
CSB-EP388992STO
CSB-BP388992STO
CSB-MP388992STO
CSB-EP388992STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 33 kDa chaperonin (hslO) CSB-YP388993STO
CSB-EP388993STO
CSB-BP388993STO
CSB-MP388993STO
CSB-EP388993STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP388994STO
CSB-EP388994STO
CSB-BP388994STO
CSB-MP388994STO
CSB-EP388994STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Probable molybdopterin-guanine dinucleotide biosynthesis protein A (mobA) CSB-YP388995STO
CSB-EP388995STO
CSB-BP388995STO
CSB-MP388995STO
CSB-EP388995STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Probable GTP-binding protein EngB (engB) CSB-YP388996STO
CSB-EP388996STO
CSB-BP388996STO
CSB-MP388996STO
CSB-EP388996STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Protein smg homolog (smg) CSB-YP388997STO
CSB-EP388997STO
CSB-BP388997STO
CSB-MP388997STO
CSB-EP388997STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Protein-export protein secB (secB) CSB-YP388998STO
CSB-EP388998STO
CSB-BP388998STO
CSB-MP388998STO
CSB-EP388998STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Bifunctional protein GlmU (glmU) CSB-YP388999STO
CSB-EP388999STO
CSB-BP388999STO
CSB-MP388999STO
CSB-EP388999STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP389000STO
CSB-EP389000STO
CSB-BP389000STO
CSB-MP389000STO
CSB-EP389000STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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