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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia mallei HTH-type transcriptional regulator BetI (betI) CSB-YP387739BPQ
CSB-EP387739BPQ
CSB-BP387739BPQ
CSB-MP387739BPQ
CSB-EP387739BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei NADH-quinone oxidoreductase subunit B 2 (nuoB2) CSB-YP387740BPQ
CSB-EP387740BPQ
CSB-BP387740BPQ
CSB-MP387740BPQ
CSB-EP387740BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP387741BPQ
CSB-EP387741BPQ
CSB-BP387741BPQ
CSB-MP387741BPQ
CSB-EP387741BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP387742BPQ
CSB-EP387742BPQ
CSB-BP387742BPQ
CSB-MP387742BPQ
CSB-EP387742BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP387743BPQ
CSB-EP387743BPQ
CSB-BP387743BPQ
CSB-MP387743BPQ
CSB-EP387743BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Integration host factor subunit beta (ihfB) CSB-YP387744BPQ
CSB-EP387744BPQ
CSB-BP387744BPQ
CSB-MP387744BPQ
CSB-EP387744BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Ribosome maturation factor RimM (rimM) CSB-YP387745BPQ
CSB-EP387745BPQ
CSB-BP387745BPQ
CSB-MP387745BPQ
CSB-EP387745BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0176 protein BMA10247_0325 (BMA10247_0325) CSB-YP387746BPQ
CSB-EP387746BPQ
CSB-BP387746BPQ
CSB-MP387746BPQ
CSB-EP387746BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Uracil phosphoribosyltransferase (upp) CSB-YP387747BPQ
CSB-EP387747BPQ
CSB-BP387747BPQ
CSB-MP387747BPQ
CSB-EP387747BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Ketol-acid reductoisomerase (ilvC) CSB-YP387748BPQ
CSB-EP387748BPQ
CSB-BP387748BPQ
CSB-MP387748BPQ
CSB-EP387748BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP387749BPQ1
CSB-EP387749BPQ1
CSB-BP387749BPQ1
CSB-MP387749BPQ1
CSB-EP387749BPQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP387750BPQ
CSB-EP387750BPQ
CSB-BP387750BPQ
CSB-MP387750BPQ
CSB-EP387750BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Phosphomethylpyrimidine synthase (thiC), partial CSB-YP387751BPQ
CSB-EP387751BPQ
CSB-BP387751BPQ
CSB-MP387751BPQ
CSB-EP387751BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Putative reductase BMA10247_0692 (BMA10247_0692) CSB-YP387752BPQ
CSB-EP387752BPQ
CSB-BP387752BPQ
CSB-MP387752BPQ
CSB-EP387752BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Putative arginyl-tRNA--protein transferase (ate) CSB-YP387753BPQ
CSB-EP387753BPQ
CSB-BP387753BPQ
CSB-MP387753BPQ
CSB-EP387753BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP387754BPQ
CSB-EP387754BPQ
CSB-BP387754BPQ
CSB-MP387754BPQ
CSB-EP387754BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei tRNA pseudouridine synthase B (truB) CSB-YP387755BPQ
CSB-EP387755BPQ
CSB-BP387755BPQ
CSB-MP387755BPQ
CSB-EP387755BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Adenylosuccinate synthetase (purA) CSB-YP387756BPQ
CSB-EP387756BPQ
CSB-BP387756BPQ
CSB-MP387756BPQ
CSB-EP387756BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Protein hfq (hfq) CSB-YP387757BPQ
CSB-EP387757BPQ
CSB-BP387757BPQ
CSB-MP387757BPQ
CSB-EP387757BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP387758BPQ
CSB-EP387758BPQ
CSB-BP387758BPQ
CSB-MP387758BPQ
CSB-EP387758BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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