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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella loihica Histidine--tRNA ligase (hisS) CSB-YP388941STO
CSB-EP388941STO
CSB-BP388941STO
CSB-MP388941STO
CSB-EP388941STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP388942STO
CSB-EP388942STO
CSB-BP388942STO
CSB-MP388942STO
CSB-EP388942STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Flagellar P-ring protein (flgI) CSB-YP388943STO
CSB-EP388943STO
CSB-BP388943STO
CSB-MP388943STO
CSB-EP388943STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Putative pterin-4-alpha-carbinolamine dehydratase (Shew_1436) CSB-YP388944STO
CSB-EP388944STO
CSB-BP388944STO
CSB-MP388944STO
CSB-EP388944STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Serine/threonine transporter sstT (sstT), partial CSB-YP388945STO
CSB-EP388945STO
CSB-BP388945STO
CSB-MP388945STO
CSB-EP388945STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Protein TolB (tolB) CSB-YP388946STO
CSB-EP388946STO
CSB-BP388946STO
CSB-MP388946STO
CSB-EP388946STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Putative phosphotransferase Shew_1548 (Shew_1548) CSB-YP388947STO
CSB-EP388947STO
CSB-BP388947STO
CSB-MP388947STO
CSB-EP388947STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP388948STO
CSB-EP388948STO
CSB-BP388948STO
CSB-MP388948STO
CSB-EP388948STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Methionine--tRNA ligase (metG), partial CSB-YP388949STO
CSB-EP388949STO
CSB-BP388949STO
CSB-MP388949STO
CSB-EP388949STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica 50S ribosomal protein L32 (rpmF) CSB-YP388950STO
CSB-EP388950STO
CSB-BP388950STO
CSB-MP388950STO
CSB-EP388950STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Biosynthetic arginine decarboxylase (speA), partial CSB-YP388951STO
CSB-EP388951STO
CSB-BP388951STO
CSB-MP388951STO
CSB-EP388951STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP388952STO
CSB-EP388952STO
CSB-BP388952STO
CSB-MP388952STO
CSB-EP388952STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Biotin synthase (bioB) CSB-YP388953STO
CSB-EP388953STO
CSB-BP388953STO
CSB-MP388953STO
CSB-EP388953STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Dihydroorotate dehydrogenase (quinone) CSB-YP388954STO
CSB-EP388954STO
CSB-BP388954STO
CSB-MP388954STO
CSB-EP388954STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Deoxyguanosinetriphosphate triphosphohydrolase-like protein (Shew_1912) CSB-YP388955STO
CSB-EP388955STO
CSB-BP388955STO
CSB-MP388955STO
CSB-EP388955STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Cytidylate kinase (cmk) CSB-YP388956STO
CSB-EP388956STO
CSB-BP388956STO
CSB-MP388956STO
CSB-EP388956STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Serine--tRNA ligase (serS) CSB-YP388957STO
CSB-EP388957STO
CSB-BP388957STO
CSB-MP388957STO
CSB-EP388957STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Electron transport complex protein RnfA (rnfA), partial CSB-YP388958STO1
CSB-EP388958STO1
CSB-BP388958STO1
CSB-MP388958STO1
CSB-EP388958STO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Electron transport complex protein RnfE (rnfE), partial CSB-YP388959STO1
CSB-EP388959STO1
CSB-BP388959STO1
CSB-MP388959STO1
CSB-EP388959STO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella loihica Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP388960STO
CSB-EP388960STO
CSB-BP388960STO
CSB-MP388960STO
CSB-EP388960STO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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