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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Actinobacillus pleuropneumoniae serotype 5b Proline--tRNA ligase (proS), partial CSB-YP387939AUF
CSB-EP387939AUF
CSB-BP387939AUF
CSB-MP387939AUF
CSB-EP387939AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP387940AUF
CSB-EP387940AUF
CSB-BP387940AUF
CSB-MP387940AUF
CSB-EP387940AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP387941AUF
CSB-EP387941AUF
CSB-BP387941AUF
CSB-MP387941AUF
CSB-EP387941AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP387942AUF
CSB-EP387942AUF
CSB-BP387942AUF
CSB-MP387942AUF
CSB-EP387942AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Met repressor (metJ) CSB-YP387943AUF
CSB-EP387943AUF
CSB-BP387943AUF
CSB-MP387943AUF
CSB-EP387943AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 5'-nucleotidase surE (surE) CSB-YP387944AUF
CSB-EP387944AUF
CSB-BP387944AUF
CSB-MP387944AUF
CSB-EP387944AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b D-tyrosyl-tRNA (Tyr) deacylase CSB-YP387945AUF
CSB-EP387945AUF
CSB-BP387945AUF
CSB-MP387945AUF
CSB-EP387945AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b UPF0761 membrane protein APL_1950 (APL_1950), partial CSB-YP387946AUF1
CSB-EP387946AUF1
CSB-BP387946AUF1
CSB-MP387946AUF1
CSB-EP387946AUF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 50S ribosomal protein L28 (rpmB) CSB-YP387947AUF
CSB-EP387947AUF
CSB-BP387947AUF
CSB-MP387947AUF
CSB-EP387947AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP387948AUF
CSB-EP387948AUF
CSB-BP387948AUF
CSB-MP387948AUF
CSB-EP387948AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b ATP phosphoribosyltransferase (hisG) CSB-YP387949AUF
CSB-EP387949AUF
CSB-BP387949AUF
CSB-MP387949AUF
CSB-EP387949AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP387950BPU
CSB-EP387950BPU
CSB-BP387950BPU
CSB-MP387950BPU
CSB-EP387950BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Ribonuclease P protein component (rnpA) CSB-YP387951BPU
CSB-EP387951BPU
CSB-BP387951BPU
CSB-MP387951BPU
CSB-EP387951BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei tRNA modification GTPase MnmE (mnmE) CSB-YP387952BPU
CSB-EP387952BPU
CSB-BP387952BPU
CSB-MP387952BPU
CSB-EP387952BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP387953BPU
CSB-EP387953BPU
CSB-BP387953BPU
CSB-MP387953BPU
CSB-EP387953BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP387954BPU
CSB-EP387954BPU
CSB-BP387954BPU
CSB-MP387954BPU
CSB-EP387954BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP387955BPU
CSB-EP387955BPU
CSB-BP387955BPU
CSB-MP387955BPU
CSB-EP387955BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP387956BPU
CSB-EP387956BPU
CSB-BP387956BPU
CSB-MP387956BPU
CSB-EP387956BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Arginine--tRNA ligase (argS), partial CSB-YP387957BPU
CSB-EP387957BPU
CSB-BP387957BPU
CSB-MP387957BPU
CSB-EP387957BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei C4-dicarboxylate transport protein (dctA), partial CSB-YP387958BPU
CSB-EP387958BPU
CSB-BP387958BPU
CSB-MP387958BPU
CSB-EP387958BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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