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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pyrobaculum calidifontis 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP387839PZV
CSB-EP387839PZV
CSB-BP387839PZV
CSB-MP387839PZV
CSB-EP387839PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis UPF0282 protein Pcal_1546 (Pcal_1546) CSB-YP387840PZV
CSB-EP387840PZV
CSB-BP387840PZV
CSB-MP387840PZV
CSB-EP387840PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis DNA-directed RNA polymerase subunit P (rpoP) CSB-YP387841PZV
CSB-EP387841PZV
CSB-BP387841PZV
CSB-MP387841PZV
CSB-EP387841PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis D-tyrosyl-tRNA (Tyr) deacylase CSB-YP387842PZV
CSB-EP387842PZV
CSB-BP387842PZV
CSB-MP387842PZV
CSB-EP387842PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis 50S ribosomal protein L21e (rpl21e) CSB-YP387843PZV
CSB-EP387843PZV
CSB-BP387843PZV
CSB-MP387843PZV
CSB-EP387843PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis UPF0218 protein Pcal_1764 (Pcal_1764) CSB-YP387844PZV
CSB-EP387844PZV
CSB-BP387844PZV
CSB-MP387844PZV
CSB-EP387844PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Uridylate kinase (pyrH) CSB-YP387845PZV
CSB-EP387845PZV
CSB-BP387845PZV
CSB-MP387845PZV
CSB-EP387845PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis 50S ribosomal protein L40e (rpl40e) CSB-YP387846PZV
CSB-EP387846PZV
CSB-BP387846PZV
CSB-MP387846PZV
CSB-EP387846PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Alanine--tRNA ligase (alaS), partial CSB-YP387847PZV
CSB-EP387847PZV
CSB-BP387847PZV
CSB-MP387847PZV
CSB-EP387847PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Glutamyl-tRNA reductase 2 (hemA2) CSB-YP387848PZV
CSB-EP387848PZV
CSB-BP387848PZV
CSB-MP387848PZV
CSB-EP387848PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Tyrosine--tRNA ligase (tyrS) CSB-YP387849PZV
CSB-EP387849PZV
CSB-BP387849PZV
CSB-MP387849PZV
CSB-EP387849PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis 50S ribosomal protein L30e (rpl30e) CSB-YP387850PZV
CSB-EP387850PZV
CSB-BP387850PZV
CSB-MP387850PZV
CSB-EP387850PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis UPF0179 protein Pcal_2106 (Pcal_2106) CSB-YP387851PZV
CSB-EP387851PZV
CSB-BP387851PZV
CSB-MP387851PZV
CSB-EP387851PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Transcription factor E (tfe) CSB-YP387852PZV
CSB-EP387852PZV
CSB-BP387852PZV
CSB-MP387852PZV
CSB-EP387852PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Protein MraZ (mraZ) CSB-YP387853AUF
CSB-EP387853AUF
CSB-BP387853AUF
CSB-MP387853AUF
CSB-EP387853AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP387854AUF
CSB-EP387854AUF
CSB-BP387854AUF
CSB-MP387854AUF
CSB-EP387854AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP387855AUF
CSB-EP387855AUF
CSB-BP387855AUF
CSB-MP387855AUF
CSB-EP387855AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Ribonuclease PH (rph) CSB-YP387856AUF
CSB-EP387856AUF
CSB-BP387856AUF
CSB-MP387856AUF
CSB-EP387856AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 3-deoxy-manno-octulosonate cytidylyltransferase 1 (kdsB1) CSB-YP387857AUF
CSB-EP387857AUF
CSB-BP387857AUF
CSB-MP387857AUF
CSB-EP387857AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Cell division protein ZapA (zapA) CSB-YP387858AUF
CSB-EP387858AUF
CSB-BP387858AUF
CSB-MP387858AUF
CSB-EP387858AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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