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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodobacter sphaeroides (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP388440RIJ
CSB-EP388440RIJ
CSB-BP388440RIJ
CSB-MP388440RIJ
CSB-EP388440RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L27 (rpmA) CSB-YP388441RIJ
CSB-EP388441RIJ
CSB-BP388441RIJ
CSB-MP388441RIJ
CSB-EP388441RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Heme A synthase (ctaA), partial CSB-YP388442RIJ
CSB-EP388442RIJ
CSB-BP388442RIJ
CSB-MP388442RIJ
CSB-EP388442RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Electron transport complex protein RnfB (rnfB) CSB-YP388443RIJ
CSB-EP388443RIJ
CSB-BP388443RIJ
CSB-MP388443RIJ
CSB-EP388443RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Inositol 2-dehydrogenase (iolG) CSB-YP388444RIJ
CSB-EP388444RIJ
CSB-BP388444RIJ
CSB-MP388444RIJ
CSB-EP388444RIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides ATP synthase subunit c 2 (atpE2), partial CSB-YP388445RIJ1
CSB-EP388445RIJ1
CSB-BP388445RIJ1
CSB-MP388445RIJ1
CSB-EP388445RIJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Chromosomal replication initiator protein DnaA (dnaA) CSB-YP388446MOL
CSB-EP388446MOL
CSB-BP388446MOL
CSB-MP388446MOL
CSB-EP388446MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Putative S-adenosyl-L-methionine-dependent methyltransferase Mjls_0079 (Mjls_0079) CSB-YP388447MOL
CSB-EP388447MOL
CSB-BP388447MOL
CSB-MP388447MOL
CSB-EP388447MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Dihydroxy-acid dehydratase (ilvD), partial CSB-YP388448MOL
CSB-EP388448MOL
CSB-BP388448MOL
CSB-MP388448MOL
CSB-EP388448MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Deoxycytidine triphosphate deaminase (dcd) CSB-YP388449MOL
CSB-EP388449MOL
CSB-BP388449MOL
CSB-MP388449MOL
CSB-EP388449MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Chaperone protein DnaK (dnaK), partial CSB-YP388450MOL
CSB-EP388450MOL
CSB-BP388450MOL
CSB-MP388450MOL
CSB-EP388450MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Acetate kinase (ackA) CSB-YP388451MOL
CSB-EP388451MOL
CSB-BP388451MOL
CSB-MP388451MOL
CSB-EP388451MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP388452MOL
CSB-EP388452MOL
CSB-BP388452MOL
CSB-MP388452MOL
CSB-EP388452MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP388453MOL
CSB-EP388453MOL
CSB-BP388453MOL
CSB-MP388453MOL
CSB-EP388453MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 50S ribosomal protein L33 1 (rpmG1) CSB-YP388454MOL
CSB-EP388454MOL
CSB-BP388454MOL
CSB-MP388454MOL
CSB-EP388454MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 50S ribosomal protein L11 (rplK) CSB-YP388455MOL
CSB-EP388455MOL
CSB-BP388455MOL
CSB-MP388455MOL
CSB-EP388455MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP388456MOL
CSB-EP388456MOL
CSB-BP388456MOL
CSB-MP388456MOL
CSB-EP388456MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 50S ribosomal protein L4 (rplD) CSB-YP388457MOL
CSB-EP388457MOL
CSB-BP388457MOL
CSB-MP388457MOL
CSB-EP388457MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 50S ribosomal protein L22 (rplV) CSB-YP388458MOL
CSB-EP388458MOL
CSB-BP388458MOL
CSB-MP388458MOL
CSB-EP388458MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 50S ribosomal protein L5 (rplE) CSB-YP388459MOL
CSB-EP388459MOL
CSB-BP388459MOL
CSB-MP388459MOL
CSB-EP388459MOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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