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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bovine Peptidyl-prolyl cis-trans isomerase E (PPIE) CSB-YP389242BO
CSB-EP389242BO
CSB-BP389242BO
CSB-MP389242BO
CSB-EP389242BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana (E,E)-alpha-farnesene synthase, partial CSB-YP389243DOA
CSB-EP389243DOA
CSB-BP389243DOA
CSB-MP389243DOA
CSB-EP389243DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Tetrahydromethanopterin S-methyltransferase subunit B (mtrB), partial CSB-YP389244MNP1
CSB-EP389244MNP1
CSB-BP389244MNP1
CSB-MP389244MNP1
CSB-EP389244MNP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Signal recognition 54 kDa protein (srp54) CSB-YP389245MNP
CSB-EP389245MNP
CSB-BP389245MNP
CSB-MP389245MNP
CSB-EP389245MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S19P (rps19p) CSB-YP389246MNP
CSB-EP389246MNP
CSB-BP389246MNP
CSB-MP389246MNP
CSB-EP389246MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0212 protein MmarC5_0068 (MmarC5_0068) CSB-YP389247MNP
CSB-EP389247MNP
CSB-BP389247MNP
CSB-MP389247MNP
CSB-EP389247MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable cobyrinic acid A,C-diamide synthase (cobB) CSB-YP389248MNP
CSB-EP389248MNP
CSB-BP389248MNP
CSB-MP389248MNP
CSB-EP389248MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM) CSB-YP389249MNP
CSB-EP389249MNP
CSB-BP389249MNP
CSB-MP389249MNP
CSB-EP389249MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis tRNA (cytidine (56)-2'-O)-methyltransferase CSB-YP389250MNP
CSB-EP389250MNP
CSB-BP389250MNP
CSB-MP389250MNP
CSB-EP389250MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L6P (rpl6p) CSB-YP389251MNP
CSB-EP389251MNP
CSB-BP389251MNP
CSB-MP389251MNP
CSB-EP389251MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L24P (rpl24p) CSB-YP389252MNP
CSB-EP389252MNP
CSB-BP389252MNP
CSB-MP389252MNP
CSB-EP389252MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Protein translation factor SUI1 homolog (MmarC5_0172) CSB-YP389253MNP
CSB-EP389253MNP
CSB-BP389253MNP
CSB-MP389253MNP
CSB-EP389253MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Elongation factor 1-beta (ef1b) CSB-YP389254MNP
CSB-EP389254MNP
CSB-BP389254MNP
CSB-MP389254MNP
CSB-EP389254MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 5-formaminoimidazole-4-carboxamide-1- (beta)-D-ribofuranosyl 5'-monophosphate synthetase CSB-YP389255MNP
CSB-EP389255MNP
CSB-BP389255MNP
CSB-MP389255MNP
CSB-EP389255MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S7P (rps7p) CSB-YP389256MNP
CSB-EP389256MNP
CSB-BP389256MNP
CSB-MP389256MNP
CSB-EP389256MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L30e (rpl30e) CSB-YP389257MNP
CSB-EP389257MNP
CSB-BP389257MNP
CSB-MP389257MNP
CSB-EP389257MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribose 1,5-bisphosphate isomerase (MmarC5_0226) CSB-YP389258MNP
CSB-EP389258MNP
CSB-BP389258MNP
CSB-MP389258MNP
CSB-EP389258MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S4P (rps4p) CSB-YP389259MNP
CSB-EP389259MNP
CSB-BP389259MNP
CSB-MP389259MNP
CSB-EP389259MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L10e (rpl10e) CSB-YP389260MNP
CSB-EP389260MNP
CSB-BP389260MNP
CSB-MP389260MNP
CSB-EP389260MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Glutamyl-tRNA (Gln) amidotransferase subunit E, partial CSB-YP389261MNP
CSB-EP389261MNP
CSB-BP389261MNP
CSB-MP389261MNP
CSB-EP389261MNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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